Caloric restriction alters the hormonal profile and testicular metabolome, resulting in alterations of sperm head morphology
Caloric restriction alters the hormonal profile and testicular metabolome, resulting in alterations of sperm head morphology
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DOI：
10.1152/ajpendo.00355.2019
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发表时间：
2020-01-01
影响因子：
5.1
通讯作者：
Alves, Marco G.
中科院分区：
文献类型：
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作者：
Martins, Ana D.;Jarak, Ivana;Alves, Marco G.
能量平衡对所有生理过程都至关重要。因此，当能量摄入量低时，可能会对健康产生负面影响，包括生殖功能。我们建议研究热量限制（CR）是否会改变睾丸代谢状况并最终改变精子质量。将雄性Wistar大鼠（n = 12）随机分为CR组，其喂食的卡路里比体重匹配的自由进食动物（对照组）少30%。分析循环激素谱、睾丸胰高血糖素样肽-1（GLP-1）、生长激素释放肽和瘦素受体表达以及精子参数。分别用核磁共振和蛋白质印迹法研究睾丸代谢产物丰度和糖酵解相关酶。在睾丸组织和精子中分析氧化应激标记物。测定睾丸线粒体复合物和线粒体生物合成的表达。CR诱导体重沿着变化以及GLP-1、生长激素释放肽和瘦素循环水平改变。在睾丸中，CR导致受体表达的变化，随后激素水平的变化;修改睾丸代谢组，特别是氨基酸含量;减少睾丸和精子中氧化应激诱导的损伤，尽管精子头部缺陷增加。总之，CR诱导体重变化，改变循环激素谱和睾丸代谢组，并增加精子头部缺陷。最终，我们的数据强调CR的条件可能会损害男性生育能力。
Energy homeostasis is crucial for all physiological processes. Thus, when there is low energy intake, negative health effects may arise, including in reproductive function. We propose to study whether caloric restriction (CR) changes testicular metabolic profile and ultimately sperm quality. Male Wistar rats (n = 12) were randomized into a CR group fed with 30% fewer calories than weight-matched, ad libitum-fed animals (control group). Circulating hormonal profile, testicular glucagon-like peptide-1 (GLP-1), ghrelin and leptin receptors expression, and sperm parameters were analyzed. Testicular metabolite abundance and glycolysis-related enzymes were studied by NMR and Western blot, respectively. Oxidative stress markers were analyzed in testicular tissue and spermatozoa. Expressions of mitochondrial complexes and mitochondrial biogenesis in testes were determined. CR induced changes in body weight along with altered GLP-1, ghrelin, and leptin circulating levels. In testes, CR led to changes in receptor expression that followed those of the hormone levels; modified testicular metabolome, particularly amino acid content; and decreased oxidative stress-induced damage in testis and spermatozoa, although sperm head defects increased. In sum, CR induced changes in body weight, altering circulating hormonal profile and testicular metabolome and increasing sperm head defects. Ultimately, our data highlight that conditions of CR may compromise male fertility.