Cartilage and bone cells do not participate in skeletal regeneration in Ambystoma mexicanum limbs
Cartilage and bone cells do not participate in skeletal regeneration in Ambystoma mexicanum limbs
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DOI：
10.1016/j.ydbio.2016.05.032
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发表时间：
2016-08-01
影响因子：
2.7
通讯作者：
Gardiner, David M.
中科院分区：
文献类型：
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作者：
McCusker, Catherine D.;Diaz-Castillo, Carlos;Gardiner, David M.
墨西哥蝾螈是少数能够在受伤的成年肢体中再生完整骨骼元素的四足动物之一。骨骼（骨和软骨）是否在骨骼再生的模式和贡献中起作用目前还没有解决。我们测试了图案形成的诱导，对细胞增殖的影响，以及骨骼组织（软骨，骨和骨膜）对再生蝾螈肢体的贡献。我们发现，表达GFP的转基因供体的骨组织移植物不能诱导模式形成，也不能促进新再生的骨骼。另一方面，骨膜组织移植物具有这两种活性。这些观察结果表明，骨骼组织并不有助于骨骼元素的再生;相反，这些结构是由非骨骼结缔组织来源的细胞形成的。(C)2016 Elsevier Inc. All rights reserved.
The Mexican Axolotl is one of the few tetrapod species that is capable of regenerating complete skeletal elements in injured adult limbs. Whether the skeleton (bone and cartilage) plays a role in the patterning and contribution to the skeletal regenerate is currently unresolved. We tested the induction of pattern formation, the effect on cell proliferation, and contributions of skeletal tissues (cartilage, bone, and periosteum) to the regenerating axolotl limb. We found that bone tissue grafts from transgenic donors expressing GFP fail to induce pattern formation and do not contribute to the newly regenerated skeleton. Periosteum tissue grafts, on the other hand, have both of these activities. These observations reveal that skeletal tissue does not contribute to the regeneration of skeletal elements; rather, these structures are patterned by and derived from cells of non-skeletal connective tissue origin. (C) 2016 Elsevier Inc. All rights reserved.