Localization of corticotropin-releasing factor, urotensin I, and CRF-binding protein gene expression in the brain of the zebrafish, Danio rerio
Localization of corticotropin-releasing factor, urotensin I, and CRF-binding protein gene expression in the brain of the zebrafish, Danio rerio
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DOI：
10.1002/cne.21332
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发表时间：
2007-06-10
影响因子：
2.5
通讯作者：
Bernier, Nicholas J.
中科院分区：
文献类型：
--
作者：
Alderman, Sarah L.;Bernier, Nicholas J.
我们目前的了解促肾上腺皮质激素释放因子（CRF）系统在硬骨鱼脑中的分布是有限的免疫组化研究和缺乏完整的转录分布图的限制。本研究采用原位杂交定位和比较CRF，尾加压素I（UI），CRF结合蛋白（CRF-BP）在成年斑马鱼（Danio rerio）脑中的表达。所有三种肽都位于视前区，室周下丘脑和顶盖区，和背侧部分的三叉神经运动核。CRF和UI都在端脑背侧的几个核团中表达，而CRF和CRF-BP都在端脑腹侧的腹侧核团中表达。CRF和CRF-BP仅在嗅球和上级中缝核中表达，而UI仅在乳头体、内侧纵束核、被盖背核、瓣外侧核和脚间核中观察到。这项研究的一个主要发现是CRF-BP与其配体的一般区域重叠，并且倾向于与CRF而不是UI串联表达。总体而言，在这项研究中概述的mRNA表达模式支持压力相关的神经内分泌，自主神经和行为功能，一般归因于脊椎动物CRF系统，并建议一些独特的功能作用，CRF和UI在硬骨鱼类大脑。
Our current understanding of the corticotropin-releasing factor (CRF) system distribution in the teleost brain is restricted by limited immunohistochemical studies and a lack of complete transcriptional distribution maps. The present study used in situ hybridization to localize and compare CRF, urotensin I (UI), and CRF-binding protein (CRF-BP) expression in the brain of adult zebrafish (Danio rerio). All three peptides were localized in the preoptic area, periventricular hypothalamic and tectal regions, and dorsal part of the trigeminal motor nucleus. CRF and UI were both expressed in several nuclei of the dorsal telencephalon, whereas CRF and CRF-BP were both expressed in the ventral nucleus of the ventral telencephalon. Sole expression of CRF and CRF-BP was apparent in the olfactory bulbs and superior raphe nucleus, respectively, whereas only UI was observed in the corpus mamillare, nucleus of the medial longitudinal fascicle, dorsal tegmental nucleus, nucleus lateralis valvulae, and nucleus interpeduncularis. A major finding of this study was the general regional overlapping of CRF-BP with its ligands and a tendency to be expressed in tandem with CRF rather than UI. Overall, the mRNA expression patterns outlined in this study support the stress-related neuroendocrine, autonomic, and behavioral functions generally ascribed to the vertebrate CRF system and suggest some unique functional roles for CRF and UI in the teleost brain.