S-Nitroso-N-acetylpenicillamine (SNAP) Impregnated Silicone Foley Catheters: A Potential Biomaterial/Device To Prevent Catheter-Associated Urinary Tract Infections.
S-Nitroso-N-acetylpenicillamine (SNAP) Impregnated Silicone Foley Catheters: A Potential Biomaterial/Device To Prevent Catheter-Associated Urinary Tract Infections.
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DOI：
10.1021/acsbiomaterials.5b00032
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发表时间：
2015-06-08
影响因子：
5.8
通讯作者：
Meyerhoff ME
中科院分区：
文献类型：
--
作者：
Colletta A;Wu J;Wo Y;Kappler M;Chen H;Xi C;Meyerhoff ME
尿Foley导尿管用于住院患者的管理，并与尿路感染(UTIs)的高发生率相关。一氧化氮(NO)有效地抑制微生物生物膜的形成，这是导管相关性尿路感染(CAUTIs)的主要原因。在这里，商业硅胶Foley导管通过溶剂溶胀法注入S-亚硝基-N-乙酰-D-青霉胺(SNAP)，SNAP是一种合成的NO供体，在与低吸水率聚合物混合时表现出长期的NO释放和稳定性。该导管在生理条件下1个月以上不产生表面通量，最小限度地减少SNAP渗漏。这些生物医学设备被证明在3、7和14天内显著减少了引起CAUTIs的微生物(表皮葡萄球菌和奇异变形杆菌)在导管管表面的生物膜形成。毒性评估表明，SNAP浸渍的导管是完全生物相容的，因为使用公认的小鼠成纤维细胞系毒性模型，导管管的提取物在3分分级标准中得0分。因此，植入SNAP的硅胶Foley导尿管可能会提供一种有效的策略，极大地减少医院内CAUTI的发生。
Urinary Foley catheters are utilized for management of hospitalized patients and are associated with high rates of urinary tract infections (UTIs). Nitric oxide (NO) potently inhibits microbial biofilm formation, which is the primary cause of catheter associated UTIs (CAUTIs). Herein, commercial silicone Foley catheters are impregnated via a solvent swelling method with S-nitroso-N-acetyl-D-penicillamine (SNAP), a synthetic NO donor that exhibits long-term NO release and stability when incorporated into low water-uptake polymers. The proposed catheters generate NO surface-fluxes >0.7 × 10–10 mol min–1 cm–2 for over one month under physiological conditions, with minimal SNAP leaching. These biomedical devices are demonstrated to significantly decrease formation of biofilm on the surface of the catheter tubings over 3, 7, and 14 day periods by microbial species (Staphylococcus epidermidis and Proteus mirabilis) commonly causing CAUTIs. Toxicity assessment demonstrates that the SNAP-impregnated catheters are fully biocompatible, as extracts of the catheter tubings score 0 on a 3-point grading scale using an accepted mouse fibroblast cell-line toxicity model. Consequently, SNAP-impregnated silicone Foley catheters can likely provide an efficient strategy to greatly reduce the occurrence of nosocomial CAUTIs.