Characterization of the Q.Ymym region on wheat chromosome 2D associated with wheat yellow mosaic virus resistance
Characterization of the Q.Ymym region on wheat chromosome 2D associated with wheat yellow mosaic virus resistance
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DOI：
10.1111/pbr.12759
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发表时间：
2020-02
期刊：
影响因子：
2
通讯作者：
F. Kobayashi;Hisayo Kojima;Tsuyoshi Tanaka;M. Saito;C. Kiribuchi‐Otobe;Toshiki Nakamura
中科院分区：
文献类型：
--
作者：
F. Kobayashi;Hisayo Kojima;Tsuyoshi Tanaka;M. Saito;C. Kiribuchi‐Otobe;Toshiki Nakamura
小麦黄花叶病是由小麦黄花叶病毒（Wheat Yellow Mosaic Virus，WYMV）引起的一种严重的冬小麦病害。在2D染色体上的两个标记Xcfd233和Xgwm349之间的43.6cM连锁区间上，定位了日本小麦品种Yumechikara的一个抗WYMV主效QTL，命名为Q.Ymym。我们能够在该块内获得两个重组体，这有助于减小连接块的大小。对“中国春”（CS）的假分子序列分析表明，原始的Q.Ymym43.6cM区域相当于68.5Mb，克隆的Q.Ymym27.3Mb。与CS序列相比，Q. Ymyn区的序列特征是独特的，这可能导致了该区块内重组率低。在其他WYMV抗性品种中检测到Q.Ymymhaplotype块，但在本研究中使用的感病品种中未检测到。Q. Ymymm区域的独特序列结构允许开发用于标记辅助选择的共显性标记。
Yellow mosaic disease, caused bywheat yellow mosaic virus(WYMV), is one of the most serious diseases of winter wheat in Japan and China. A single major QTL for WYMV resistance in the Japanese wheat variety 'Yumechikara', designatedQ.Ymym, has been mapped on a 43.6 cM linkage block between the two markersXcfd233andXgwm349on chromosome 2D. We were able to obtain two recombinants within the block, which facilitated reducing the size of the linkage block. The pseudomolecule sequence of 'Chinese Spring' (CS) indicated that the originalQ.Ymymregion of 43.6 cM corresponded to 68.5 Mb and the narrowedQ.Ymymregion represents a size of 27.3 Mb. The sequence features of theQ.Ymymregion were unique in comparison with CS sequences, which may have led to the low recombination rate within the block. TheQ.Ymymhaplotype block was detected in other WYMV‐resistant varieties but not in the susceptible varieties used in this study. The unique sequence structure of theQ.Ymymregion allowed the development of co‐dominant markers for use in marker‐assisted selection.