Analysis of gene expression in Ca2+-dependent activator protein for secretion 2 (Cadps2) knockout cerebellum using GeneChip and KEGG pathways
Analysis of gene expression in Ca2+-dependent activator protein for secretion 2 (Cadps2) knockout cerebellum using GeneChip and KEGG pathways
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DOI：
10.1016/j.neulet.2016.12.068
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发表时间：
2017-02
影响因子：
2.5
通讯作者：
T. Sadakata;Y. Shinoda;Y. Ishizaki;T. Furuichi
中科院分区：
文献类型：
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作者：
T. Sadakata;Y. Shinoda;Y. Ishizaki;T. Furuichi
在小鼠小脑中，Ca 2+依赖性分泌激活蛋白2（CADPS 2，CAPS 2）参与调节致密核心囊泡（DCV）的分泌，DCV含有神经肽，包括脑源性神经营养因子（BDNF）和神经营养因子-3（NT-3）。为了了解Capds 2缺失所引起的分子影响，我们使用基因芯片微阵列和KEGG Pathway数据库分析了Capds 2KO小脑中的基因表达谱。在芯片上显示的22，690个基因中，有1211个（5.34%）观察到显著差异表达。胞吐相关基因（Stx 5a、Syt 6）、编码分泌蛋白（Fgf 2、Fgf 4、Edn 2）和突触蛋白（Grin 2b、Gabbr 1）的基因、神经营养因子信号相关基因（Sos 1、Shc 1、Traf 6、Psen 2）和Rett综合征基因（Mecp 2）的表达水平发生显著变化。总之，这些结果表明，由Capds 2缺失引起的基因表达失调可能导致发育缺陷和/或病理症状，导致自闭症样表型。
In the mouse cerebellum, Ca2+-dependent activator protein for secretion 2 (CADPS2, CAPS2) is involved in regulated secretion from dense-core vesicles (DCVs), which contain neuropeptides including brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3).Capds2knockout (KO) mice show impaired cerebellar development in addition to autistic-like behavioral phenotypes. To understand the molecular impact caused by loss ofCapds2, we analyzed gene expression profiles in theCapds2KO cerebellum using a GeneChip microarray and the KEGG Pathway database. Significant differential expression was observed in 1211 of 22,690 (5.34%) genes represented on the chip. The expression levels of exocytosis-related genes (Stx5a, Syt6), genes encoding secretory (Fgf2, Fgf4, Edn2) and synaptic proteins (Grin2b, Gabbr1), neurotrophin signaling-associated genes (Sos1, Shc1, Traf6, Psen2), and a gene for Rett syndrome (Mecp2) were significantly changed. Taken together, these results suggest that deregulated gene expression caused by loss ofCapds2may cause developmental deficits and/or pathological symptoms, resulting in autistic-like phenotypes.