Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum.
Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum.
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DOI：
10.1186/scrt97
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发表时间：
2012-02-14
影响因子：
7.5
通讯作者：
Domenech J
中科院分区：
文献类型：
--
作者：
Ben Azouna N;Jenhani F;Regaya Z;Berraeis L;Ben Othman T;Ducrocq E;Domenech J
间充质干细胞（MSC）是能够分化成几种间充质谱系的多能细胞，经典地来源于骨髓（BM），但可能来源于脐带血（UCB）。虽然它们正在成为再生医学的良好工具，但它们通常需要在补充胎牛血清（FBS）的培养基中扩增。出于安全原因，最近有人提出用人血小板裂解物（HPL）作为替代品，但目前尚不清楚这种补充剂如何影响扩增的MSC的性质。在本研究中，我们比较了各种培养基结合自体HPL与或不与FBS对人骨髓源性MSC的表型，增殖和功能（分化，细胞因子分泌谱）特征的影响。尽管成脂和成骨标记物表达较少，但在补充有HPL的培养基中培养的MSC沿着成骨细胞、成脂细胞、成软骨细胞和血管平滑肌谱系完全分化沿着分化。对成骨分化过程中表达的特定特异性蛋白质（钙敏感受体（CaSR）和甲状旁腺激素受体（PTHR））的分析显示，在用HPL培养的MSC的任何诱导之前，它们在D0时以高百分比（10%HPL）降低。细胞因子剂量显示增殖能力和白细胞介素（IL）-6和IL-8分泌明显增加。该研究表明，MSC可以在补充有HPL的培养基中扩增，所述HPL可以完全替代FBS。补充HPL的培养基不仅保留了它们的表型以及它们的分化能力，而且通过增加它们的生长速率来缩短培养时间。
Mesenchymal stem cells (MSCs) are multipotent cells able to differentiate into several mesenchymal lineages, classically derived from bone marrow (BM) but potentially from umbilical cord blood (UCB). Although they are becoming a good tool for regenerative medicine, they usually need to be expanded in fetal bovine serum (FBS)-supplemented media. Human platelet lysate (HPL) has recently been proposed as substitute for safety reasons, but it is not yet clear how this supplement influences the properties of expanded MSCs. In the present study, we compared the effect of various media combining autologous HPL with or without FBS on phenotypic, proliferative and functional (differentiation, cytokine secretion profile) characteristics of human BM-derived MSCs. Despite less expression of adipogenic and osteogenic markers, MSCs cultured in HPL-supplemented media fully differentiated along osteoblastic, adipogenic, chondrogenic and vascular smooth muscle lineages. The analyses of particular specific proteins expressed during osteogenic differentiation (calcium-sensing receptor (CaSR) and parathormone receptor (PTHR)) showed their decrease at D0 before any induction for MSC cultured with HPL mostly at high percentage (10%HPL). The cytokine dosage showed a clear increase of proliferation capacity and interleukin (IL)-6 and IL-8 secretion. This study shows that MSCs can be expanded in media supplemented with HPL that can totally replace FBS. HPL-supplemented media not only preserves their phenotype as well as their differentiation capacity, but also shortens culture time by increasing their growth rate.
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影响因子：
5.6
作者：
Doucet, C;Ernou, I;Lataillade, JJ
通讯作者：
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作者：
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