Forkhead transcription factor HFH-4 expression is temporally related to ciliogenesis
Forkhead transcription factor HFH-4 expression is temporally related to ciliogenesis
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DOI：
10.1165/ajrcmb.21.2.3691
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发表时间：
1999-08-01
影响因子：
6.4
通讯作者：
Brody, SL
中科院分区：
文献类型：
--
作者：
Blatt, EN;Yan, XH;Brody, SL
叉头/翼螺旋转录因子家族的成员以组织特异性模式表达，并在发育和细胞分化中发挥关键作用。叉头家族成员肝细胞核因子3/叉头同源物4（HFH-4）的表达已通过RNA印迹分析和原位杂交定位于肺的近端气道（气管、支气管和细支气管），在小鼠胚胎日（E14.5）开始，并存在于脉络丛、室管膜细胞、输卵管和睾丸中。我们假设HFH-4信使RNA的限制性表达表明这些组织具有共同的功能，因此HFH-4具有细胞特异性作用。因此，产生抗HFH-4抗体并用于蛋白质表达的细胞特异性定位以开始鉴定HFH-4的功能。我们发现HFH-4在近端气道纤毛上皮细胞中表达，而不是克拉拉细胞或肺泡上皮细胞。HFH-4在鼻和鼻窦的纤毛上皮细胞中也有表达。脉络丛室管膜和输卵管。在发育中的小鼠肺中，HFH-4表达最初在E15.5的气道上皮细胞中检测到，在纤毛出现之前，并且在后期阶段定位于具有纤毛的上皮细胞。在精巢中，精子细胞中HFH-4的表达与鞭毛的阶段特异性产生相一致。HFH-4表达与纤毛和鞭毛发育的时间关系，以及在纤毛上皮细胞中的限制性表达？提示该转录因子在上皮细胞中纤毛细胞表型的调节和维持中具有作用。
Members of the forkhead/winged-helix family of transcription factors are expressed in tissue-specific patterns and play critical roles in development and cell differentiation. The expression of forkhead family member hepatocyte nuclear factor-3/forkhead homologue 4 (HFH-4) has been localized by RNA-blot analysis and in situ hybridization to the proximal airway of the lung (trachea, bronchi, and bronchioles) with onset at mouse embryonic day (E 14.5 and is present in the choroid plexus, ependymal cells, oviduct, and testis. We hypothesized that the restricted expression of HFH-4 messenger RNA suggests a function common to these tissues and therefore a cell-specific role for HFH-4. Accordingly, an anti-HFH-4 antibody was generated and used for cell-specific localization of protein expression to begin to identify the functions of HFH-4. We found HFH-4 expression in proximal airway ciliated epithelial cells, but not Clara cells or alveolar epithelial cells. HFH-4 was also expressed in ciliated epithelial cells of the nose and paranasal sinuses. choroid plexus, ependyma, and oviduct. In developing mouse lung, HFH-4 expression was initially detected in airway epithelial cells at E15.5, before the appearance of cilia, and at later stages was localized to epithelial cells with cilia. In the testis, HFH-4 expression in spermatids was coincident with stage-specific generation of flagella. The temporal relationship of HFH-4 expression to the development of cilia and flagella, and the restricted expression in ciliated epithelial cells? suggest that this transcription factor has a role in regulation and maintenance of the ciliated cell phenotype in epithelial cells.