Thermoreversible laminin-functionalized hydrogel for neural tissue engineering.
Thermoreversible laminin-functionalized hydrogel for neural tissue engineering.
复制标题
用于神经组织工程的热可逆层粘连蛋白功能化水凝胶。
DOI：
10.1002/jbm.a.30638
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
LaPlaca,MichelleC
中科院分区：
文献类型：
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作者：
Stabenfeldt,SarahE;Garcia,AndresJ;LaPlaca,MichelleC
中枢神经系统 (CNS) 的创伤性损伤会引发细胞死亡和传入神经阻滞，从而可能激活一系列细胞和网络紊乱。这些事件通常导致形成由坏死组织和/或充满液体的空腔组成的不规则形状病变。组织工程代表了一种有前途的治疗受损神经组织的策略。为了促进组织工程系统的微创递送，热可逆聚合物是一种有吸引力的支架候选物。我们通过将层粘连蛋白-1 (LN) 与甲基纤维素 (MC)（一种热响应水凝胶）连接，开发了一种用于神经组织工程的生物活性支架。通过高碘酸钠氧化基础 MC 链以增加 MC 束缚能力。 LN 上的伯胺基团和氧化的 MC 链的羰基之间的希夫碱反应促进了蛋白质固定。免疫测定表明 LN 的束缚浓度为每毫克 MC 1.6 ± 0.5 ng LN。不同 MC-LN 结构的流变学测量表明 MC 组成和 MC 处理对溶液-凝胶化转变温度有依赖性影响。对原代大鼠皮质神经元进行的细胞测定表明，与基础和氧化 MC 相比，LN 功能化 MC 的细胞粘附和活力增强。这种生物粘附热响应支架可以为受伤的中枢神经系统组织提供强大的递送载体，用于神经细胞移植策略。 © 2006 Wiley periodicals, Inc. J Biomed Mater Res，2006
Traumatic injury to the central nervous system (CNS) triggers cell death and deafferentation, which may activate a cascade of cellular and network disturbances. These events often result in the formation of irregularly shaped lesions comprised of necrotic tissue and/or a fluid‐filled cavity. Tissue engineering represents a promising treatment strategy for the injured neural tissue. To facilitate minimally invasive delivery of a tissue engineered system, a thermoreversible polymer is an attractive scaffold candidate. We have developed a bioactive scaffold for neural tissue engineering by tethering laminin‐1 (LN) to methylcellulose (MC), a thermoresponsive hydrogel. The base MC chain was oxidized via sodiumm‐periodate to increase MC tethering capacity. Protein immobilization was facilitated by a Schiff base reaction between primary amine groups on LN and the carbonyl groups of the oxidized MC chain. Immunoassays demonstrated tethering of LN at 1.6 ± 0.5 ng of LN per milligram of MC. Rheological measurements for different MC–LN constructs indicated MC composition‐ and MC treatment‐dependent effects on solution–gelation transition temperature. Cellular assays with primary rat cortical neurons demonstrated enhanced cell adhesion and viability on LN‐functionalized MC when compared with base and oxidized MC. This bioadhesive thermoresponsive scaffold may provide a robust delivery vehicle to injured CNS tissue for neural cell transplantation strategies. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006