IDENTIFICATION AND PURIFICATION OF A CELL-SURFACE GLYCOPROTEIN MEDIATING INTERCELLULAR-ADHESION IN EMBRYONIC AND ADULT TISSUE
IDENTIFICATION AND PURIFICATION OF A CELL-SURFACE GLYCOPROTEIN MEDIATING INTERCELLULAR-ADHESION IN EMBRYONIC AND ADULT TISSUE
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DOI：
10.1016/0092-8674(83)90379-3
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
BUCK, CA
中科院分区：
文献类型：
--
作者：
DAMSKY, CH;RICHA, J;BUCK, CA
针对MCF-7人乳腺癌细胞脱落到无血清培养基中的物质的抗血清（抗SFM II）破坏了小鼠乳腺肿瘤上皮细胞（MMTE）中的细胞-细胞相互作用。从MCF-7乳腺癌细胞的SEM中纯化了80 kd [千道尔顿]糖蛋白（GP 80），其阻断抗SFM II的活性。抗SFM II还抑制8细胞小鼠胚胎的致密化，并且纯化的GP 80阻断该反应。抗纯化GP 80的抗血清（抗GP 80）具有抗SFM II显示的所有粘附破坏活性。它识别SFM中80 kd处的一条带和上皮细胞但不识别成纤维细胞的洗涤剂提取物中的120 kd带。在免疫荧光研究中，它仅限于培养的上皮细胞中细胞-细胞相互作用的位点。一种120 kd的细胞表面糖蛋白，其中等形式为. apprx。80 kd既不是物种特异性的，也不是组织特异性的，在哺乳动物发育的早期阶段表达，并且在上皮细胞上发现。
An antiserum against material shed into serum-free medium by MCF-7 human mammary carcinoma cells (anti-SFM II) disrupts cell-cell interactions in murine mammary tumor epithelial cells (MMTE). An 80 kd [kilodalton] glycoprotein (GP80) was purified from SEM of MCF-7 mammary carcinoma cells that blocks the activity of anti-SFM II. Anti-SFM II also inhibits compaction of 8-cell mouse embryos, and purified GP80 blocks this reaction. An antiserum against purified GP80 (anti-GP80) has all adhesion-disrupting activities displayed by anti-SFM II. It recognizes one band at 80 kd in SFM and a 120 kd band in detergent extracts of epithelial but not fibroblastic cells. In immunofluorescence studies it is restricted to sites of cell-cell interaction in cultured epithelial cells. A cell surface glycoprotein of 120 kd, the medium form of which is .apprx. 80 kd, which is neither species nor tissue specific, is expressed at early stages of mammalian development and is found on epithelia.