Gα14 is a candidate mediator of sweet/umami signal transduction in the posterior region of the mouse tongue
Gα14 is a candidate mediator of sweet/umami signal transduction in the posterior region of the mouse tongue
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DOI：
10.1016/j.bbrc.2008.09.035
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发表时间：
2008-11-21
影响因子：
3.1
通讯作者：
Kusakabe, Yuko
中科院分区：
文献类型：
--
作者：
Shindo, Yoichiro;Miura, Hirohito;Kusakabe, Yuko
味蛋白是味觉细胞中表达的Got亚基，是甜味、鲜味和苦味信号转导的关键分子。然而，以往的研究表明，味觉调节蛋白对舌后部甜味/鲜味反应的贡献小于舌前部，这意味着存在另一个G α亚基介导甜味/鲜味信号转导。在此，我们提出G α q家族成员G α 14作为候选介体. G α 14被发现在我们的Substance全长cDNA文库来自小鼠轮廓乳头（CV）和CV和叶状乳头的味觉细胞的子集中表达，但不是在菌状乳头和软腭。在CV中，G α 14与甜味/鲜味受体T1 r3共表达，但不与gustducin共表达。这些结果表明，G α 14在舌后部区域的甜味/鲜味信号转导中的重要作用。(c)2008年爱思唯尔公司All rights reserved.
Gustducin, a Got Subunit expressed in taste cells, is known as a key molecule for sweet, Umami and bitter taste signal transduction. However, previous studies demonstrated that the contribution of gustducin to the sweet/umami responses in the posterior region of the tongue is less than that ill the anterior region, implying the existence of another G alpha subunit mediating sweet/umami taste signal transductional. Here, we Propose G alpha 14, a member of G alpha q family, as the candidate mediator. G alpha 14 was found in our Subtracted full-length cDNA library derived from mouse circumvallate papillae (CV) and expressed in a subset of taste cells in CV and foliate papillae, but not in fungiform papillae and soft palate. G alpha 14 was co-expressed with T1r3, a sweet/umami taste receptor, but not with gustducin in CV. These results suggest the important roles of G alpha 14 in sweet/umami taste signal transduction in the posterior region of the tongue. (c) 2008 Elsevier Inc. All rights reserved.