Electrical stimulation of human embryonic stem cells: cardiac differentiation and the generation of reactive oxygen species.
Electrical stimulation of human embryonic stem cells: cardiac differentiation and the generation of reactive oxygen species.
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DOI：
10.1016/j.yexcr.2009.08.015
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发表时间：
2009-12-10
影响因子：
3.7
通讯作者：
Vunjak-Novakovic G
中科院分区：
文献类型：
--
作者：
Serena E;Figallo E;Tandon N;Cannizzaro C;Gerecht S;Elvassore N;Vunjak-Novakovic G
外源性电场对小鼠胚胎干细胞心肌分化和活性氧的产生有一定的影响。在这项工作中，我们探讨了电场刺激对ROS的产生和来自人胚胎干细胞（hESC，系H13）的胚状体（EBs）的心脏发生的影响，使用定制的电刺激生物反应器。电化学阻抗谱（EIS）和电流分析的生物反应器系统的电性能进行了表征。电极材料（不锈钢，氮化钛涂层的钛，钛），刺激长度（1秒和90秒）和年龄的EB在电刺激开始（4和8天）的影响进行了研究，相对于ROS的产生。所施加的电场的振幅为1 V/mm。观察到不锈钢电极的ROS生成速率最高，信号持续时间为90 s，和4天的EB。值得注意的是，通过将EB与1 nM H2 O2孵育实现了相当的ROS生成。自发性收缩、肌钙蛋白T的表达及其肌节组织证明了这些EB的心脏分化。这些结果表明，电刺激通过与细胞内ROS产生相关的机制在hESC的心脏分化中起作用。
Exogenous electric fields have been implied in cardiac differentiation of mouse embryonic stem cells and the generation of reactive oxygen species (ROS). In this work, we explored the effects of electrical field stimulation on ROS generation and cardiogenesis in embryoid bodies (EBs) derived from human embryonic stem cells (hESC, line H13), using a custom-built electrical-stimulation bioreactor. Electrical properties of the bioreactor system were characterized by electrochemical impedance spectroscopy (EIS) and analysis of electrical currents. The effects of the electrode material (stainless steel, titanium-nitride coated titanium, titanium), length of stimulus (1 s and 90 s) and age of EBs at the onset of electrical stimulation (4 and 8 days) were investigated with respect to ROS generation. The amplitude of the applied electrical field was 1 V/mm. The highest rate of ROS generation was observed for stainless steel electrodes, for signal duration of 90 s, and 4-days old EBs. Notably, comparable ROS generation was achieved by incubation of EBs with 1 nM H2O2. Cardiac differentiation in these EBs was evidenced by spontaneous contractions, expression of Troponin T, and its sarcomeric organization. These results imply that electrical stimulation plays a role in cardiac differentiation of hESCs, through mechanisms associated with the intracellular generation of ROS.
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影响因子：
3.8
作者：
MACDONALD, JR
通讯作者：
MACDONALD, JR
影响因子：
4.8
作者：
Setsukinai, K;Urano, Y;Nagano, T
通讯作者：
Nagano, T
DOI：
10.1073/pnas.0407817101
发表时间：
2004-12-28
影响因子：
11.1
作者：
Radisic, M;Park, H;Vunjak-Novakovic, G
通讯作者：
Vunjak-Novakovic, G
影响因子：
3.7
作者：
Sauer, H;Neukirchen, W;Wartenberg, M
通讯作者：
Wartenberg, M
影响因子：
3.3
作者：
Li, Jian;Stouffs, Michael;Jaconi, Marisa E.
通讯作者：
Jaconi, Marisa E.