Oncogene-related growth factors and growth factor receptors in human malignant glioma-derived cell lines.
Oncogene-related growth factors and growth factor receptors in human malignant glioma-derived cell lines.
复制标题
人恶性胶质瘤衍生细胞系中癌基因相关的生长因子和生长因子受体。
DOI：
10.1007/bf00149378
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发表时间：
1989
影响因子：
3.9
通讯作者：
Williams,LT
中科院分区：
文献类型：
--
作者：
Harsh4th,GR;Rosenblum,ML;Williams,LT
致癌基因诱导细胞恶性转化。两个致癌基因与编码有丝分裂生长因子（v-sisto PDGF基因）和有丝分裂生长因子受体（v- erbb Bto EGF受体基因）的基因密切相关。我们研究了来自恶性胶质瘤的细胞产生与细胞表面受体结合的有丝分裂因子的可能性，该因子的激活可能导致过度刺激细胞增殖。所有六种被测试的细胞系都在培养基中分泌刺激DNA合成的因子。一种细胞系分泌的因子被表征并发现与PDGR相似，11种细胞系中有6种通过结合和受体自磷酸化实验证明具有PDGF受体。通过结合和受体自磷酸化实验，六种细胞系中有六种具有EGF受体。一种细胞系中EGF受体的极高水平可能反映了与7号染色体异常相关的Boncogene的过度表达。
Oncogenes induce malignant transformation of cells. Two oncogenes are closely related to genes coding for a mitogenic growth factor (v-sisto the PDGF gene) and a receptor for a mitogenic growth factor (v-erb Bto the EGF receptor gene). We studied the possibility that cells derived from malignant gliomas produce mitogenic factors that bind to cell surface receptors, the activation of which could lead to excessive stimulation of cell proliferation. All six cell lines tested secrete into their medium factors that stimulate DNA synthesis. The factor secreted by one cell line was characterized and found to resemble PDGR Six of 11 cell lines had receptors for PDGF demonstrable by binding and receptor autophosphorylation assays. Six of six cell lines tested had EGF receptors demonstrable by binding and receptor autophosphorylation experiments. The extremely high levels of EGF receptor in one cell line may reflect excessive expression of theerb Boncogene associated with abnormalities of chromosome 7 that occur in this cell line.