A composite chitosan-gelatin bi-layered, biomimetic macroporous scaffold for blood vessel tissue engineering
A composite chitosan-gelatin bi-layered, biomimetic macroporous scaffold for blood vessel tissue engineering
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DOI：
10.1016/j.carbpol.2016.09.095
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发表时间：
2017-02-10
影响因子：
11.2
通讯作者：
Pillay, Viness
中科院分区：
文献类型：
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作者：
Badhe, Ravindra V.;Bijukumar, Divya;Pillay, Viness
采用溶剂浇铸-共颗粒浸出的方法设计了一种具有双层管状结构的基于壳聚糖-明胶大孔水凝胶的复合支架。该支架由无孔外层隐藏的内部大孔层组成，模拟血管的 3D 基质，具有细胞粘附和增殖作用。采用 SEM、FTIR、DSC、XRD、孔隙率测定、流变学和质地分析评估支架的形态、物理化学、物理机械和生物耐久性能。还研究了溶菌酶存在下的液体吸收和生物降解。使用接种到支架上的人真皮成纤维细胞 (HDF-a) 分析细胞附着和增殖，并通过 MTT 测定、SEM 和共聚焦显微镜进行评估。结果表明，该支架具有理想的拉伸强度= 95.81 +/- 11 kPa，断裂伸长率112.5 +/- 13%，孔隙率为82%，孔隙在100至230μm之间，第16天体外生物降解率为50%，成纤维细胞在20天内增殖。这些结果表明支架可能是血管组织工程的优良管状原型。 (C) 2016 Elsevier Ltd. 保留所有权利。
A composite chitosan-gelatin macroporous hydrogel-based scaffold with bi-layered tubular architecture was engineered by solvent casting-co-particulate leaching.The scaffold constituted an inner macroporous layer concealed by a non-porous outer layer mimicking the 3D matrix of blood vessels with cellular adhesion and proliferation. The scaffold was evaluated for its morphological, physicochemical, physicomechanical and biodurability properties employing SEM, FTIR, DSC, XRD, porositometry, rheology and texture analysis. The fluid uptake and biodegradation in the presence of lysozymes was also investigated. Cellular attachment and proliferation was analysed using human dermal fibroblasts (HDF-a) seeded onto the scaffold and evaluated by MTT assay, SEM, and confocal microscopy. Results demonstrated that the scaffold had a desirable tensile strength = 95.81 +/- 11 kPa, elongation at break 112.5 +/- 13%, porosity 82% and pores between 100 and 230 mu m, 50% in vitro biodegradation at day 16 and proliferated fibroblasts over 20 days. These results demonstrate that scaffold may be an excellent tubular archetype for blood vessel tissue engineering. (C) 2016 Elsevier Ltd. All rights reserved.