Direct monitoring of organic vapours with amperometric enzyme gas sensors
Direct monitoring of organic vapours with amperometric enzyme gas sensors
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DOI：
10.1016/j.bios.2009.10.022
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发表时间：
2010-02-15
影响因子：
12.6
通讯作者：
Moos, Ralf
中科院分区：
文献类型：
--
作者：
Haemmerle, Martin;Hilgert, Karin;Moos, Ralf
在这项研究中，电流型酶气体传感器直接监测的有机蒸气（甲醛，乙醇和苯酚）提出了使用示例性的不同的传感策略：NADH检测，H（2）O（2）检测和直接底物回收，分别。所提出的传感器配置允许选择性地、连续地、在线地监测有机蒸气，而无需对分析物进行预先积累或采样。气体样品作为水溶液上方的顶部空间提供。根据文献中给出的相应亨利常数，由室温下溶液中的浓度计算气相中的浓度。所用的酶是来自恶臭假单胞菌的NAD依赖性甲醛脱氢酶[EC 1.2.1.46]、来自巴斯德毕赤酵母的醇氧化酶[EC 1.1.3.13]和来自蘑菇的酪氨酸酶[EC 1.14.18.1]。传感器中使用的气体扩散工作电极基于多孔疏水PTFE膜（暴露的几何电极面积：1.77 cm（2）），覆盖有金、铂或石墨/Teflon多孔层。甲醛的检测限、灵敏度和测量范围分别为34 μ M（6.5 ppb）、117 nA/mM和0.46-66.4 mM，乙醇的检测限、灵敏度和测量范围分别为9.9 μ M（55 ppb）、3.43 μ A/mM和0.1-30 mM，苯酚的检测限、灵敏度和测量范围分别为0.89 μ M（0.36 ppb）、2.4 μ A/mM和0.01-1 mM。还测定了其他传感器特性，如响应时间和稳定性：t（90%）（甲醛：4.5 min;乙醇：69 s;苯酚：27 min），永久暴露时的稳定性（甲醛：63%，15 h@2.62 mM;乙醇：86%，18 h@1 mM;苯酚：86%，16.5 h@0.1 mM）。(c)2009爱思唯尔有限公司版权所有。
In this study, amperometric enzyme gas sensors for direct monitoring of organic vapours (formaldehyde, ethanol and phenol) are presented using exemplarily different sensing strategies: NADH detection, H(2)O(2) detection and direct substrate recycling, respectively. The presented sensor configurations allow the selective, continuous, online monitoring of organic vapours without prior accumulation or sampling of the analyte. The gaseous samples are provided as headspace above aqueous solutions. The concentration in the gas phase was calculated from the concentration in solution at room temperature according to the respective Henry constants given in the literature. The enzymes employed are NAD-dependent formaldehyde dehydrogenase [EC 1.2.1.46] from Pseudomonas putida, alcohol oxidase [EC 1.1.3.13] from Pichia pastoris, and tyrosinase [EC 1.14.18.1] from mushroom. The gas diffusion working electrodes used in the sensors are based on a porous, hydrophobic PTFE membrane (exposed geometric electrode area: 1.77 cm(2)) covered with a porous layer of gold, platinum or graphite/Teflon. Detection limit, sensitivity, and measuring range are 34 mu M (6.5 ppb), 117 nA/mM, and 0.46-66.4 mM for formaldehyde, 9.9 mu M (55 ppb), 3.43 mu A/mM, and 0.1-30 mM for ethanol, and 0.89 mu M (0.36 ppb), 2.4 mu A/mM, and 0.01-1 mM for phenol, respectively. Further sensor characteristics such as response time and stability are also determined: t(90%) (formaldehyde: 4.5 min; ethanol: 69 s; phenol: 27 min), stability at permanent exposure (formaldehyde: 63%, 15h @ 2.62 mM; ethanol: 86%, 18 h @ 1 mM; phenol: 86%,16.5 h @ 0.1 mM). (c) 2009 Elsevier B.V. All rights reserved.