Distribution and origin of VIP-, SP-, and phospholipase Cβ2 - immunoreactive nerves in the tongue of the bullfrog, Rana catesbeiana.
Distribution and origin of VIP-, SP-, and phospholipase Cβ2 - immunoreactive nerves in the tongue of the bullfrog, Rana catesbeiana.
复制标题
牛蛙舌头中 VIP-、SP- 和磷脂酶 Cβ2- 免疫反应神经的分布和起源。
DOI：
10.1002/ar.23334
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Yagasaki H
中科院分区：
文献类型：
--
作者：
Tadokoro O;Ando H;Kawahara I;Asanuma N;Okumura M;Kitagawa J;Kondo E;Yagasaki H
以往的研究在蛙舌内发现了一些对血管活性肠肽（VIP）有免疫反应的神经节细胞。最近的一项研究报告了大量的这种细胞，以及从这些细胞释放P物质（SP）的可能性。本研究的目的是研究牛蛙舌中VIP和SP免疫反应神经的分布、起源和共定位。猫。此外，还观察了SP和磷脂酶Cβ2（PLCβ2）在舌和颈静脉神经节中的共定位。VIP免疫反应见于单极细胞，这些细胞在粘膜下层和肌肉层的神经束中稀疏。VIP免疫反应性细胞的密度约为4.8个细胞/mm 3。它们的纤维终止于菌状乳头的上皮基底层附近。在VIP免疫反应细胞中未观察到SP免疫反应，但在颈静脉神经节的假单极细胞中观察到SP免疫反应。SP纤维终止于接近自由表面，显示纺锤形和纽扣状轮廓。舌咽神经切断导致舌中VIP免疫反应阳性细胞的持续存在和SP免疫反应阳性纤维的消失。舌和颈静脉神经节中SP免疫反应性与PLCβ 2共表达。上皮味盘细胞未见PLCβ 2免疫反应。提示VIP神经纤维起源于舌的单极细胞，而SP和PLCβ 2纤维起源于颈静脉神经节内释放SP的假单极细胞。Anat Rec，299：929-942，2016.© 2016 Wiley Periodicals，Inc.
Previous studies have found a few intralingual ganglionic cells that were immunoreactive to vasoactive intestinal polypeptide (VIP) in the frog. A recent study reported a large number of such cells, and the possibility of the release of substance P (SP) from these. The aim of the present study was to investigate the distribution, origin, and colocalization of VIP‐ and SP‐ immunoreactive nerves in the tongue of the bullfrog,R. catesbeiana. In addition, the study also examined the colocalization of SP and phospholipase Cβ2(PLCβ2) in the tongue and jugular ganglion. VIP immunoreactivity was seen in unipolar cells that were sparse in nerve bundles in the submucosal and muscle layers. The density of VIP‐immunoreactive cells was approximately 4.8 cells/mm3. Their fibers terminated in the vicinity of the epithelial basal layer of the fungiform papillae. SP immunoreactivity was not seen in the VIP‐immunoreactive cells, but was observed in pseudounipolar cells in the jugular ganglion. The SP fibers terminated close to the free surface, showing spindle‐ and button‐like profiles. Transection of glossopharyngeal nerve resulted in the persistence of VIP‐immunoreactive cells and the disappearance of SP‐immunoreactive fibers in the tongue. SP immunoreactivity was co‐expressed with PLCβ2in both the tongue and jugular ganglia. No PLCβ2immunoreactivity was seen in cells comprising the epithelial taste disk. These findings indicate that the origin of VIP nerve fibers are unipolar cells in the tongue, and SP and PLCβ2fibers originate from pseudounipolar cells that may be able to release SP primarily in the jugular ganglion. Anat Rec, 299:929–942, 2016. © 2016 Wiley Periodicals, Inc.