Sulfur amino acids in methionine-restricted rats: Hyperhomocysteinemia
Sulfur amino acids in methionine-restricted rats: Hyperhomocysteinemia
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DOI：
10.1016/j.nut.2009.09.017
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发表时间：
2010-11-01
期刊：
影响因子：
4.4
通讯作者：
Perrone, Carmen E.
中科院分区：
文献类型：
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作者：
Elshorbagy, Amany K.;Valdivia-Garcia, Maria;Perrone, Carmen E.
目标：饮食蛋氨酸限制Fischer-344大鼠有利地影响内脏脂肪量、胰岛素敏感性、代谢参数和寿命。然而，蛋氨酸限制对血清蛋氨酸及其下游含硫氨基酸的影响知之甚少。我们研究了喂食蛋氨酸限制饮食3个月的雄性Fischer-344大鼠的血清含硫氨基酸谱。方法和结果：使用串联质谱法，我们观察到血清中蛋氨酸、胱硫醚、半胱氨酸，与对照组相比，甲硫氨酸限制大鼠中的牛磺酸（P < 0.001）和同型半胱氨酸升高2.5倍（P < 0.001）。结论：这表明高半胱氨酸转硫作用可能受到蛋氨酸限制的抑制，并且甲硫氨酸限制的某些作用可能由甲硫氨酸下游的含硫氨基酸的变化介导。爱思唯尔公司出版
Objective: Dietary methionine restriction in Fischer-344 rats favorably influences visceral fat mass, insulin sensitivity, metabolic parameters, and longevity. However, little is known about the effects of methionine restriction on serum methionine and its downstream sulfur amino acids. We investigated the serum sulfur amino acid profile of male Fischer-344 rats fed a methionine-restricted diet for 3 mo.Methods and results: Using tandem mass spectrometry, we observed marked reduction in serum concentrations of methionine, cystathionine, cysteine, and taurine in methionine-restricted rats compared with control (P < 0.001) and a 2.5-fold elevation of homocysteine (P < 0.001).Conclusion: This suggests that homocysteine trans-sulfuration may be inhibited by methionine restriction, and that some of the effects of methionine restriction may be mediated by changes in sulfur amino acids downstream of methionine. Published by Elsevier Inc.