Frequencies of PRNP gene polymorphisms in Vietnamese dairy cattle for potential association with BSE
Frequencies of PRNP gene polymorphisms in Vietnamese dairy cattle for potential association with BSE
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DOI：
10.1111/j.1863-2378.2008.01119.x
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发表时间：
2008-06-01
影响因子：
2.4
通讯作者：
Tamura, Y.
中科院分区：
文献类型：
--
作者：
Muramatsu, Y.;Sakemi, Y.;Tamura, Y.
自2004年以来，牛海绵状脑病（BSE）易感性与牛朊蛋白基因（PRNP）内插入/缺失（ins/del; indel）多态性频率之间存在显著相关性。在这项研究中，我们调查了206头越南奶牛和7头日本疯牛病感染牛的PRNP中两个可变位点内的indel多态性频率，即启动子区的23-bp indel多态性（23 indel）和内含子1区的12-bp indel多态性（12 indel）。在越南奶牛中，被认为与疯牛病易感性相关的23 indel位点的del等位基因和del/del基因型多态性的频率分布显着较高，而12 indel位点的del等位基因和del/del基因型多态性的频率已报道赋予疯牛病易感性，显着较低。我们提供的证据表明，越南奶牛有一个独特的遗传背景，在PRNP基因与牛或公牛以前在其他国家报道。
Since 2004, significant associations between bovine spongiform encephalopathy (BSE) susceptibility in cattle and frequencies of insertion/deletion (ins/del; indel) polymorphisms within the bovine prion protein gene (PRNP) have been reported. In this study, we investigated the frequencies of indel polymorphisms within two variable sites, a 23-bp indel polymorphism in the promoter region (23indel) and a 12-bp indel polymorphism in intron 1 region (12indel), in the PRNP in 206 Vietnamese dairy cattle and seven Japanese BSE-affected cattle. In Vietnamese dairy cattle, the frequency distributions of del allele and del/del genotypic polymorphisms in the 23indel site, which are thought to be associated with BSE susceptibility, were significantly higher, whereas the frequencies of del allelic and del/del genotypic polymorphisms in the 12indel site, which have been reported to confer BSE susceptibility, were significantly lower. We have provided evidence that Vietnamese dairy cattle have a unique genetic background in the PRNP gene in comparison with cattle or sires previously reported in other countries.