Protein Nanocapsule Weaved with Enzymatically Degradable Polymeric Network
Protein Nanocapsule Weaved with Enzymatically Degradable Polymeric Network
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DOI：
10.1021/nl902935b
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发表时间：
2009-12-01
期刊：
影响因子：
10.8
通讯作者：
Tang, Yi
中科院分区：
文献类型：
--
作者：
Gu, Zhen;Yan, Ming;Tang, Yi
利用界面聚合形成的茧状聚合物纳米胶囊可对靶蛋白进行功能封装。纳米胶囊由多肽交联，多肽可以被蛋白酶水解，蛋白质货物在蛋白酶上被释放。蛋白酶介导的降解过程可以通过修饰具有光敏感性的肽交联剂在时空上进行控制。我们通过细胞质向癌细胞递送诱导凋亡的caspase-3来证明这种方法的实用性。
Target proteins can be functionally encapsulated using a cocoon-like polymeric nanocapsule formed by interfacial polymerization. The nanocapsule is cross-linked by peptides that can be proteolyzed by proteases upon which the protein cargo is released. The protease-mediated degradation process can be controlled in a spatiotemporal fashion through modification of the peptide cross-linker with photolabile moieties. We demonstrate the utility of this approach through the cytoplasmic delivery of the apoptosis inducing caspase-3 to cancer cells.