International Test Results for Objective Lens Quality, Resolution, Spectral Accuracy and Spectral Separation for Confocal Laser Scanning Microscopes
International Test Results for Objective Lens Quality, Resolution, Spectral Accuracy and Spectral Separation for Confocal Laser Scanning Microscopes
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DOI：
10.1017/s1431927613013470
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发表时间：
2013-12-01
影响因子：
2.8
通讯作者：
Brown, Claire M.
中科院分区：
文献类型：
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作者：
Cole, Richard W.;Thibault, Marc;Brown, Claire M.
除了改善跨仪器的一致性外，作为提高图像重复性和保真度的持续努力的一部分，我们建议使用四种不同的仪器质量测试来增强我们先前报告的测试。这四项测试评估了以下方面：(1)物镜质量，(2)分辨率，(3)来自光谱探测器的波长信息的准确性，以及(4)光谱分离算法的准确性和质量。数据来自18个国家的55个实验室。所有测试中最大的错误来源是用户错误，用户错误可细分为未能遵守所提供的方案和不正确使用显微镜。这确实强调了在进行共聚焦显微镜实验和设备评估时适当严格的培训和勤奋的重要性。应该指出的是，不同的共聚焦显微镜制造商在质量上没有明显的差异。这些测试以及之前报道的其他测试将有助于评估共焦显微镜设备的质量，并将提供一种随着时间的推移跟踪设备性能的手段。从62%到97%的数据集通过了各种测试，证明了这些测试作为更大的性能测试团的一部分的有用性和适当性。
As part of an ongoing effort to increase image reproducibility and fidelity in addition to improving cross-instrument consistency, we have proposed using four separate instrument quality tests to augment the ones we have previously reported. These four tests assessed the following areas: (1) objective lens quality, (2) resolution, (3) accuracy of the wavelength information from spectral detectors, and (4) the accuracy and quality of spectral separation algorithms. Data were received from 55 laboratories located in 18 countries. The largest source of errors across all tests was user error which could be subdivided between failure to follow provided protocols and improper use of the microscope. This truly emphasizes the importance of proper rigorous training and diligence in performing confocal microscopy experiments and equipment evaluations. It should be noted that there was no discernible difference in quality between confocal microscope manufactures. These tests, as well as others previously reported, will help assess the quality of confocal microscopy equipment and will provide a means to track equipment performance over time. From 62 to 97% of the data sets sent in passed the various tests demonstrating the usefulness and appropriateness of these tests as part of a larger performance testing regiment.