A low-cost technique to cryo-protect and freeze rodent brains, precisely aligned to stereotaxic coordinates for whole-brain cryosectioning.
A low-cost technique to cryo-protect and freeze rodent brains, precisely aligned to stereotaxic coordinates for whole-brain cryosectioning.
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DOI：
10.1016/j.jneumeth.2013.03.004
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发表时间：
2013-09-15
影响因子：
3
通讯作者：
Mitra, Partha P.
中科院分区：
文献类型：
--
作者：
Pinskiy, Vadim;Tolpygo, Alexander S.;Jones, Jamie;Weber, Kevin;Franciotti, Neil;Mitra, Partha P.
关键词：
脑组织组织切片的一个主要挑战是在标准冠状面、水平面或矢状面中实现精确对齐。正确的对齐对于后续分析的容易性是期望的，并且是实验数据的计算配准和基于算法的量化的先决条件。我们已经开发出一种简单而低成本的啮齿动物大脑全脑冷冻切片技术，可靠地导致立体定向坐标的精确对准。该系统利用小鼠大脑的3D打印模型来创建一个定制的腔体，用于在冷冻过程中对齐和支撑大脑。相对于模具的固定边缘实现冷冻块的对准。该系统还允许同时冷冻和切片两个大脑。系统组件，程序步骤，并最终结果的例子。
A major challenge in the histological sectioning of brain tissue is achieving accurate alignment in the standard coronal, horizontal, or sagittal planes. Correct alignment is desirable for ease of subsequent analysis and is a prerequisite for computational registration and algorithm-based quantification of experimental data. We have developed a simple and low-cost technique for whole-brain cryosectioning of rodent brains that reliably results in a precise alignment of stereotactic coordinates. The system utilises a 3-D printed model of a mouse brain to create a tailored cavity that is used to align and support the brain during freezing. The alignment of the frozen block is achieved in relation to the fixed edge of the mold. The system also allows for two brains to be frozen and sectioned simultaneously. System components, procedural steps, and examples of the end results are presented.
影响因子：
4.2
作者：
Salie, Rishard;Li, Haitao;Susa, Mira
通讯作者：
Susa, Mira
影响因子：
3.2
作者：
Jiang, X;Kalajzic, Z;Rowe, DW
通讯作者：
Rowe, DW