Identification of heading date quantitative trait locus Hd6 and characterization of its epistatic interactions with Hd2 in rice using advanced backcross progeny.
Identification of heading date quantitative trait locus Hd6 and characterization of its epistatic interactions with Hd2 in rice using advanced backcross progeny.
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发表时间：
2000-02
期刊：
影响因子：
3.3
通讯作者：
Toshio Yamamoto;Hong-Xuan Lin;T. Sasaki;Masahiro Yano
中科院分区：
文献类型：
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作者：
Toshio Yamamoto;Hong-Xuan Lin;T. Sasaki;Masahiro Yano
由粳稻品种日本晴（作为轮回亲本）和籼稻品种 Kasalath（作为供体亲本）杂交衍生的回交群体 (BC(4)F(2)) 在抽穗前的天数内表现出长程变异。数量性状位点（QTL）分析显示，有两个QTL参与了这一变异，一个位于3号染色体上，命名为Hd6，另一个位于2号染色体上，命名为Hd7。通过后代测试 (BC(4)F(3))，Hd6 被精确映射为单个孟德尔因子。通过标记辅助选择，开发了带有日本晴遗传背景中 Hd6 区域 Kasalath 染色体片段的 QTL 近等基因系 (QTL-NIL)。在日长处理测试中，与轮回亲本日本晴相比，在 13.5 小时日长下，Hd6 的 QTL-NIL 显着增加了抽穗天数，表明 Hd6 控制光周期敏感性。来自 QTL-NIL 之间杂交的 F(2) 群体的 QTL 分析揭示了 Hd2 和 Hd6 之间存在上位相互作用，Hd2 是先前分析中检测到的光周期敏感性基因之一。这些 QTL-NIL（包括 Hd2 和 Hd6 基因渗入的品系）的日长处理测试也表明它们之间的光周期敏感性存在上位相互作用。
A backcrossed population (BC(4)F(2)) derived from a cross between a japonica rice variety, Nipponbare, as the recurrent parent and an indica rice variety, Kasalath, as the donor parent showed a long-range variation in days to heading. Quantitative trait loci (QTL) analysis revealed that two QTL, one on chromosome 3, designated Hd6, and another on chromosome 2, designated Hd7, were involved in this variation; and Hd6 was precisely mapped as a single Mendelian factor by using progeny testing (BC(4)F(3)). The nearly isogenic line with QTL (QTL-NIL) that carries the chromosomal segment from Kasalath for the Hd6 region in Nipponbare's genetic background was developed by marker-assisted selection. In a day-length treatment test, the QTL-NIL for Hd6 prominently increased days to heading under a 13.5-hr day length compared with the recurrent parent, Nipponbare, suggesting that Hd6 controls photoperiod sensitivity. QTL analysis of the F(2) population derived from a cross between the QTL-NILs revealed existence of an epistatic interaction between Hd2, which is one of the photoperiod sensitivity genes detected in a previous analysis, and Hd6. The day-length treatment tests of these QTL-NILs, including the line introgressing both Hd2 and Hd6, also indicated an epistatic interaction for photoperiod sensitivity between them.