A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses.
A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses.
复制标题
来自蚊子 Psorophora confinnis（双翅目：蚊科）的新连续细胞系及其对某些虫媒病毒感染的敏感性。
DOI：
--
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
G. Rey
中科院分区：
文献类型：
--
作者：
F. Bello;J. A. Rodríguez;Jesús E Escovar;V. Olano;A. Morales;Martha González;G. Rey
从蚊虫Psorophora confinnis的胚胎卵中建立了一个新的细胞系PC-0199-BR。到目前为止（2000年9月），它已经连续传代62次。这是首次报道的一个属于Psorophora属的蚊子细胞系。最初在补充有20%胎牛血清的MM/VP 12培养基中实现细胞生长;然而，随后将传代培养物适应于含有10%胎牛血清的Grace培养基。原代培养物中的细胞形态是异质性的;但后来在建立的细胞系中，主要的细胞类型是上皮样的。培养的细胞主要是二倍体（2n=6）;然而，在以后的传代中，在一小部分细胞中观察到染色体异常。核型中还测定了C和G带型。细胞系同工酶谱与取自同一群体的物种的蛹和成虫样品一致。对该细胞系进行了初步虫媒病毒易感性研究。未观察到细胞系被细菌、真菌或支原体污染的证据。
A new cell line, PC-0199-BR, was established from embryonated eggs of the mosquito Psorophora confinnis. To date (September 2000) it has had 62 continuous passages. This is the first report of a cell line of mosquitoes belonging to the genus Psorophora. Cell growth initially was achieved in the MM/VP12 medium, supplemented with 20% fetal bovine serum; however, the subcultures were later adapted to Grace's medium with 10% fetal bovine serum. Cell morphology in the primary cultures was heterogeneous; but later in the established cell line, the predominant cell type was epithelioid. Cultured cells were predominantly diploid (2n=6); however, chromosome abnormalities were observed in a small proportion of the cells in later passages. C and G band patterns were also determined in the karyotype. The cell line isozyme profiles coincided with pupae and adult samples of the species taken from the same colony. A preliminary arbovirus susceptibility study for the cell line was undertaken. No evidence was observed of contamination of the cell line with bacteria, fungi or mycoplasma.