Galectin-1 promotes an M2 macrophage response to polydioxanone scaffolds.
Galectin-1 promotes an M2 macrophage response to polydioxanone scaffolds.
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Galectin-1 促进 M2 巨噬细胞对聚二氧环己酮支架的反应。
DOI：
10.1002/jbm.a.36113
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发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
McClure MJ
中科院分区：
文献类型：
--
作者：
Abebayehu D;Spence A;Boyan BD;Schwartz Z;Ryan JJ;McClure MJ
Regulating soft tissue repair to prevent fibrosis and promote regeneration is central to creating a microenvironment conducive to soft tissue development. Macrophages play an important role in this process. Their response can be modulated using biomaterials, altering cytokine and growth factor secretion to promote regeneration. Electrospun polydioxanone (PDO) fiber scaffolds promoted an M2 phenotype when macrophages were cultured on large diameter, highly porous scaffolds, but an M1 phenotype on smaller diameter fibers. Here we investigated whether incorporation of galectin-1, an immunosuppressive protein that enhances muscle regeneration, could promote the M2 response. Galectin-1 was incorporated into large and small fiber PDO scaffolds during electrospinning. Galectin-1 incorporation increased arginase-1 and reduced iNOS and IL-6 production in mouse bone-marrow derived macrophages compared to PDO alone for both scaffold types. Inhibition of ERK mitogen activated protein kinase did not alter galectin-1 effects on arginase-1 and iNOS expression, but reversed IL-6 suppression, indicating that IL-6 is mediated by a different mechanism. Our results suggest that galectin-1 can be used to modulate macrophage commitment to a pro-regenerative M2 phenotype, which may positively impact tissue regeneration when using small diameter PDO scaffolds.
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影响因子：
4.9
作者：
Chong BF;Tseng LC;Hosler GA;Teske NM;Zhang S;Karp DR;Olsen NJ;Mohan C
通讯作者：
Mohan C
影响因子：
4
作者：
Garg, K.;Sell, S. A.;Bowlin, G. L.
通讯作者：
Bowlin, G. L.
影响因子：
4.1
作者：
Kim, Chaekyu;Shores, Lucas;Elisseeff, Jennifer H.
通讯作者：
Elisseeff, Jennifer H.
影响因子：
4.9
作者：
Mann CJ;Perdiguero E;Kharraz Y;Aguilar S;Pessina P;Serrano AL;Muñoz-Cánoves P
通讯作者：
Muñoz-Cánoves P
影响因子：
2.4
作者：
Lin, TW;Cardenas, L;Soslowsky, LJ
通讯作者：
Soslowsky, LJ