T-CELL RECEPTOR DELTA-GENE MUTANT MICE - INDEPENDENT GENERATION OF ALPHA-BETA T-CELLS AND PROGRAMMED REARRANGEMENTS OF GAMMA-DELTA TCR GENES
T-CELL RECEPTOR DELTA-GENE MUTANT MICE - INDEPENDENT GENERATION OF ALPHA-BETA T-CELLS AND PROGRAMMED REARRANGEMENTS OF GAMMA-DELTA TCR GENES
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DOI：
10.1016/0092-8674(93)90112-4
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发表时间：
1993-02-12
期刊：
影响因子：
64.5
通讯作者：
TONEGAWA, S
中科院分区：
文献类型：
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作者：
ITOHARA, S;MOMBAERTS, P;TONEGAWA, S
携带T细胞受体（TCR）γ和δ链异二聚体的T细胞首先在个体发育早期产生。它们形成在其TCR库和组织分布方面不同的不同亚群。通过基因靶向方法破坏小鼠TCR C δ基因片段导致携带TCR γ δ链的T细胞完全丧失，但对携带TCR α链的T细胞的发育几乎没有影响。TCR γ和δ基因突变小鼠的分析表明，细胞内的机制在DNA重排的水平上发挥作用的差异γ和δ基因重排，并在胎儿胸腺发育过程中的高度限制性连接序列的产生中发挥关键作用。
T cells bearing T cell receptor (TCR) gamma and delta chain heterodimers are first generated early in ontogeny. They form distinct subsets that differ in their TCR repertoires and tissue distribution. Disruption of the mouse TCR Cdelta gene segment by a gene targeting method caused the complete loss of T cells bearing TCR gammadelta chains, but had little or no effect on the development of T cells bearing TCR alphabeta chains. The analyses of TCR gamma and delta genes in the mutant mice suggest that intracellular mechanisms acting at the level of DNA rearrangement play key roles in the differential gamma and delta gene rearrangements and in the generation of the highly restricted junctional sequences during fetal thymic development.