DDX6 modulates P-body and stress granule assembly, composition, and docking.
DDX6 modulates P-body and stress granule assembly, composition, and docking.
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DDX6 调节 P 体和应力颗粒的组装、组成和对接。
DOI：
10.1083/jcb.202306022
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Parker,Roy
中科院分区：
文献类型：
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作者：
Ripin,Nina;MacedodeVasconcelos,Luisa;Ugay,DaniellaA;Parker,Roy
应激颗粒和P体是核糖核蛋白（RNP）颗粒，它们在应激反应期间由于未翻译mRNP的浓缩而积累。应激颗粒部分通过分子间RNA-RNA相互作用形成，并且可以受到RNA伴侣网络的组分的限制，其抑制RNA驱动的聚集。在这里，我们证明了DEAD盒解旋酶DDX 6，一个P-体组件，也可以限制应力颗粒的形成，独立于P-体的形成。DDX 6以ATP酶、RNA结合依赖性方式限制自身和其他RNP分配到应激颗粒中。当P-体受到限制时，通常在应激颗粒和P-体之间分配的蛋白质在应激颗粒内显示出增加的积累。此外，我们发现DDX 6，4 E-T和DCP 1A的丢失增加了P体与应力颗粒的对接，这取决于hocT 1和PAT 1B。综上所述，这些观察结果确定了DDX 6在限制应激颗粒中的新作用，并表明P体组分可以影响应激颗粒的组成和与P体的对接。
Stress granules and P-bodies are ribonucleoprotein (RNP) granules that accumulate during the stress response due to the condensation of untranslating mRNPs. Stress granules form in part by intermolecular RNA–RNA interactions and can be limited by components of the RNA chaperone network, which inhibits RNA-driven aggregation. Herein, we demonstrate that the DEAD-box helicase DDX6, a P-body component, can also limit the formation of stress granules, independent of the formation of P-bodies. In an ATPase, RNA-binding dependent manner, DDX6 limits the partitioning of itself and other RNPs into stress granules. When P-bodies are limited, proteins that normally partition between stress granules and P-bodies show increased accumulation within stress granules. Moreover, we show that loss of DDX6, 4E-T, and DCP1A increases P-body docking with stress granules, which depends on CNOT1 and PAT1B. Taken together, these observations identify a new role for DDX6 in limiting stress granules and demonstrate that P-body components can influence stress granule composition and docking with P-bodies.