Relationship between refractive index and mineral content of enamel and dentin using SS-OCT and TMR
Relationship between refractive index and mineral content of enamel and dentin using SS-OCT and TMR
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使用 SS-OCT 和 TMR 研究牙釉质和牙本质的折射率与矿物质含量的关系
DOI：
10.1117/12.908290
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hariri Ilnaz
中科院分区：
文献类型：
--
作者：
Sadr A;Nakashima S;Shimada Y;Tagami J;Sumi Y;Hariri Ilnaz
本研究的目的是利用扫描源光学相干断层扫描（SS-OCT）和横向显微放射照相（TMR）研究釉质和牙本质的折射率(n)与矿物质含量（MC） （vol %）之间的关系。牙釉质和牙本质块被划分为三个区域。每个样品的中间分区都用指甲油覆盖，以保护暴露在处理溶液中的声音区域。样品在脱盐溶液中脱盐，每周刷新一次，持续2个月。一扇窗户被涂上了耐酸清漆，另一扇窗户暴露在外；样品放在溶液中再矿化。然后将样品切片成厚度为300 ~ 400 μm的薄片，放置在金属板上，在1319 nm中心波长上用OCT捕捉声音、脱矿和再矿区域的截面图像。然后利用图像分析软件通过公式计算出n。测量后，这些样品被进一步抛光以进行TMR分析。检查OCT n与TMR MC的相关性。脱矿/再矿化处理后，相应位置的实际MC与测量的n呈显著的高度正相关(牙本质的Pearson相关系数（r)分别为0.94和0.97，牙本质的Pearson相关系数(r)分别为0.95和0.91,p < 0.05）。OCT显示了通过测量体外氮来定量分析矿物质损失或增加的潜力。由日本教育部资助的全球卓越中心（GCOE）计划，“牙齿和骨骼疾病分子科学国际研究中心”。
The aim of this work was to investigate relationship between refractive index (n) and mineral content (MC) (vol %) of enamel and dentin using swept-source optical coherence tomography (SS-OCT) and transverse microradiography (TMR). Enamel and dentin blocks were partitioned into three regions. The middle partition of each sample was covered with a nail polish to protect the sound area during exposure to the treatment solutions. Samples were demineralized in a demineralizing solution, which was refreshed once a week, for 2 months. One window was covered with acid-resistant varnish, leaving the other window exposed; the samples were placed in a solution for remineralization. Samples then were sliced into disks with thickness of 300 μm to 400 μm and placed on metal plate in order to capture cross-sectional images of sound, demineralized and remineralized regions by OCT at 1319 nm center wavelength. The n then was calculated via formula using image analysis software. Following n measurement, these specimens were further polished for the TMR analysis. Correlation between OCT n and TMR MC was examined. A significant and highly positive correlation was found between the measured n and the actual MC at the corresponding locations (Pearson correlation coefficients (r) were 0.94 and 0.97 in enamel and 0.95 and 0.91 in dentin after de-/remineralization process, respectively p < 0.05). OCT showed a potential for quantitative analysis of the mineral loss or gain by measuring of the n in vitro. Supported by the grant from the Japanese Ministry of Education, Global Center of Excellence (GCOE) Program, "International Research Center for Molecular Science in Tooth and Bone Diseases."