Dopamine measurements in molluscan ganglia and neurons using a new, sensitive assay
Dopamine measurements in molluscan ganglia and neurons using a new, sensitive assay
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使用新的灵敏测定法测量软体动物神经节和神经元中的多巴胺
DOI：
10.1111/j.1471-4159.1979.tb04602.x
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发表时间：
1979
影响因子：
4.7
通讯作者：
R. Mccaman
中科院分区：
文献类型：
--
作者：
M. McCaman;J. Ono;R. Mccaman
多巴胺是软体动物中枢神经系统中发现的主要儿茶酚胺，其作为神经递质的作用已在各种物种中进行了研究(见威尔士的评论，1972；GERSCHENFELD，1973；Kehoe&Marder，1976)。利用组织荧光技术，只有特定的神经元显示出DA的绿色荧光特征。生化研究已经证明，存在合适的生物合成酶系统和特定的摄取机制。电生理学研究已经描述了不同神经元中不同的DA受体，这些受体介导兴奋性、抑制性或混合性反应。关于DA神经递质作用的最令人信服的证据是对池塘蜗牛Planorbis kerus中含有DA的巨型神经元(GDC)及其突触后神经元的研究(见Berry&Pentreath，1978)。在这篇通讯中，我们使用了一种新的超灵敏的放射化学分析方法(MCCAMAN等a/.，提交发表)来测量几种海洋和淡水腹足类软体动物的神经节和单个神经元中的DA：Aplysia calfornica、Tritonia Diomedia、Lymneea stagnaris和Helisoma trioolis。含有可测量数量的DA的神经元仅见于淡水物种Lymneea和Helisoma的足神经节，可能与Planorbis中描述的G、D、C同源。
DOPAMINE is the principal catecholamine found in the molluscan CNS, and its role as a neurotransmitter has been studied in various species (see reviews by WELSH, 1972; GERSCHENFELD, 1973; KEHOE & MARDER, 1976). Using histofluorescence techniques, only specific neurons show a green fluorescence characteristic of DA. Biochemical studies have demonstrated the presence both of appropriate biosynthetic enzyme systems and of specific uptake mechanisms. Electrophysiological studies have described different receptors for DA in various neurons which mediate excitatory, inhibitory, or mixed responses. The most convincing evidence for a neurotransmitter role for DA was provided by studies of the giant DA-containing neuron (GDC) and its postsynaptic neurons in the pond snail, Planorbis corneus (see review by BERRY & PENTREATH, 1978). In this communication we have used a new, ultrasensitive radiochemical assay (MCCAMAN et a/., submitted for publication) to measure DA in the ganglia and in individual neurons of several marine and fresh water gastropod molluscs: Aplysia californica, Tritonia diomedia, Lymnaea stagnalis, and Helisoma trioolis. Neurons containing measurable amounts of DA were found only in the pedal ganglia of the fresh water species, Lymnaea and Helisoma and are probably homologous to the G D C described in Planorbis.