A comparative study on the electron microscopic enzymo-cytochemistry of Paramecium bursaria from light and dark cultures
A comparative study on the electron microscopic enzymo-cytochemistry of Paramecium bursaria from light and dark cultures
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DOI：
10.1078/0932-4739-00875
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发表时间：
2002-10-01
影响因子：
2.9
通讯作者：
Zhang, XM
中科院分区：
文献类型：
--
作者：
Gu, FK;Chen, L;Zhang, XM
对光培养和暗培养草履虫的超微结构和酶细胞化学进行了比较。在轻度培养的草履虫中，小球藻共生体生长正常，只有少数小球藻被消化。在暗培养的草履虫中，含有共生菌的食物空泡数量显著增加，食物空泡中的小球藻共生体逐渐退化，最终被宿主细胞消化。对光照和黑暗条件下培养草履虫的酸性磷酸酶、三磷酸腺苷、琥珀酸脱氢酶和葡萄糖-6-磷酸酶的活性进行了研究。结果表明，两种培养条件下草履虫的胞质结构和酶细胞化学反应不同。暗培养草履虫表现出较强的酶细胞化学反应活性。结果表明，在暗培养条件下，小球藻共生体的逐渐退化可以刺激寄主加强摄食和相关细胞器的消化活性；而在光培养条件下，小球藻共生体可以为寄主提供营养，并抑制寄主某些细胞器的功能活性。
Electron microscopic structure and enzymo-cytochemistry of light- and dark-cultured Paramecium bursaria was compared. in light-cultured Paramecium bursaria, the Chlorella symbionts grew normally and only a few of them were digested. In dark-cultured Paramecium bursaria, the number of food vacuoles containing symbionts increased significantly and the Chlorella symbionts in food vacuoles degenerated gradually and were finally digested by host cells. The activity of the enzymes acid phosphatase, adenosine triphosphatase, succinate dehydrogenase and glucose-6-phosphatase was studied in paramecia cultured in both light and dark conditions. The results showed that paramecia cultured under the two different conditions differed in their cytoplasmic structures and enzyme cytochemical reactions. The dark-cultured paramecia displayed stronger enzyme cytochemical reactivity. it is suggested that under dark-culture conditions, the gradual degeneration of Chlorella symbionts can stimulate their host to intensify its food uptake and the digestive activities of related organelles; whereas under the light-culture conditions, Chlorella symbionts can supply nutrition to their host and inhibit the functional activities of some organelles of their host.