Autologous mesenchymal stem cell-mediated repair of tendon
Autologous mesenchymal stem cell-mediated repair of tendon
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DOI：
10.1089/ten.1999.5.267
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发表时间：
1999-06-01
影响因子：
--
通讯作者：
Caplan, AI
中科院分区：
文献类型：
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作者：
Awad, HA;Butler, DL;Caplan, AI
从 18 只成年新西兰白兔的骨髓中分离出间充质干细胞 (MSC)。这些细胞经过培养扩增，悬浮在 I 型胶原凝胶中，并植入到供体右髌腱中手术引起的缺损处。将无细胞胶原凝胶植入左侧髌腱的相同对照缺损处。术后 4 周对修复组织进行生物力学评估 (n = 13) 和组织形态计量学评估 (n = 5)。与匹配的对照相比，MSG 介导的修复组织的最大应力、模量和应变能密度分别显着增加了 26% (p < 0.001)、18% (p < 0.01) 和 33% (p < 0.02)。定性地讲，一些 MSC 介导的修复的组织学外观似乎有微小的改善，包括肌腱细胞数量的增加以及更大且看起来更成熟的胶原纤维束。然而，在形态测量上，核纵横比（形状）或核排列（方向）没有显着的左右差异。因此，将大量间充质干细胞输送到伤口部位仅需4周即可显着改善其生物力学性能，但其微观结构却没有明显改善。
Mesenchymal stem cells (MSCs) were isolated from bone marrow of 18 adult New Zealand White rabbits. These cells were culture expanded, suspended in type I collagen gel, and implanted into a surgically induced defect in the donor's right patellar tendon. A cell-free collagen gel was implanted into an identical control defect in the left patellar tendon. Repair tissues were evaluated biomechanically (n = 13) and histomorphometrically (n = 5) at 4 weeks after surgery. Compared to their matched controls, the MSG-mediated repair tissue demonstrated significant increases of 26% (p < 0.001), 18% (p < 0.01), and 33% (p < 0.02) in maximum stress, modulus, and strain energy density, respectively. Qualitatively, there appeared to be minor improvements in the histological appearance of some of the MSC-mediated repairs, including increased number of tenocytes and larger and more mature-looking collagen fiber bundles. Morphometrically, however, there were no significant left-right differences in nuclear aspect ratio (shape) or nuclear alignment (orientation). Therefore, delivering a large number of mesenchymal stem cells to a wound site can significantly improve its biomechanical properties by only 4 weeks but produce no visible improvement in its microstructure.