LIGHT-STIMULATED TRANSCRIPTION OF GENES FOR 2 CHLOROPLAST POLYPEPTIDES IN ISOLATED PEA LEAF NUCLEI
LIGHT-STIMULATED TRANSCRIPTION OF GENES FOR 2 CHLOROPLAST POLYPEPTIDES IN ISOLATED PEA LEAF NUCLEI
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DOI：
10.1002/j.1460-2075.1982.tb01345.x
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发表时间：
1982-01-01
期刊：
影响因子：
11.4
通讯作者：
ELLIS, RJ
中科院分区：
文献类型：
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作者：
GALLAGHER, TF;ELLIS, RJ
当补充其他 3 种核苷三磷酸时，通过 Percoll 密度梯度离心从 P. sativum 的光生长和黑暗生长的叶子中分离出的细胞核将标记的 UTP 掺入 RNA 中。 RNA 是异分散的，转录本大小至少为 25S。这些转录物中包括与小麦 rRNA 和 2 种丰富的 Pisum 叶绿体多肽（核酮糖二磷酸羧化酶的小亚基）和光捕获叶绿素 a/b 结合复合物的多肽的克隆 DNA 探针杂交的序列。小亚基和光捕获复合物序列的转录在光生长的叶子的细胞核中比在黑暗生长的叶子的细胞核中更大（分别是18倍和9倍）。相比之下，核糖体基因的转录仅因在光照下生长而加倍。在黑暗生长的细胞核中转录的小亚基和光捕获复合序列在 120 分钟的追踪中不会被降解。通过将豌豆幼苗暴露在光下，刺激小亚基和光捕获复合体 mRNA 的积累是通过转录的增加而不是通过 RNA 降解的减少来介导的。
Nuclei isolated from both light-grown and dark-grown leaves of P. sativum by Percoll density gradient centrifugation incorporate labeled UTP into RNA when supplemented with the other 3 nucleoside triphosphates. The RNA is heterodisperse, with transcripts up to at least 25S in size. Among these transcripts are sequences hybridizing to cloned DNA probes for wheat rRNA and 2 abundant chloroplast polypeptides of Pisum, the small subunit of ribulose bisphosphate carboxylase and a polypeptide of the light-harvesting chlorophyll a/b binding complex. Transcription of small subunit and light-harvesting complex sequences is greater (18-fold and 9-fold, respectively) in nuclei from light-grown leaves than in nuclei from dark-grown leaves. Transcription of ribosomal genes, by contrast, is only doubled by growth in the light. Small subunit and light-harvesting complex sequences transcribed in dark-grown nuclei are not degraded in a 120 min chase. The stimulation of accumulation of small subunit and light-harvesting complex mRNA by exposure of Pisum seedlings to light is mediated by an increase in transcription rather than by a decrease in RNA degradation.