Purkinje cell loss in experimental autoimmune encephalomyelitis
Purkinje cell loss in experimental autoimmune encephalomyelitis
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DOI：
10.1016/j.neuroimage.2009.06.073
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发表时间：
2009-12-01
期刊：
影响因子：
5.7
通讯作者：
Voskuhl, Rhonda R.
中科院分区：
文献类型：
--
作者：
MacKenzie-Graham, Allan;Tiwari-Woodruff, Seema K.;Voskuhl, Rhonda R.
脑部 MRI 观察到的灰质萎缩与多发性硬化症的临床残疾和疾病持续时间具有重要相关性。本研究的目的是使用 MS 模型实验性自身免疫性脑脊髓炎 (EAE) 将神经影像学显示的脑萎缩与其潜在的神经病理学联系起来。通过收集死后高分辨率 T2 加权磁共振显微镜和主动染色的磁共振组织学图像，对 EAE 小鼠以及匹配的健康正常对照的大脑体积变化进行量化。解剖学描绘表明，与正常对照组相比，EAE 患者的整个小脑、小脑皮质和小脑皮质分子层的体积显着减小。在浦肯野细胞中检测到促凋亡标记物 caspase-3，并且在 EAE 中发现浦肯野细胞数量显着减少。交叉模态和时间相关性揭示了神经病理学上的浦肯野细胞丢失与神经影像学显示的小脑皮质分子层萎缩之间存在显着关联。这些结果证明了使用群体图谱和神经病理学相结合的方法来辨别神经退行性疾病动物模型中灰质萎缩的新见解的力量。由爱思唯尔公司出版
Gray matter atrophy observed by brain MRI is an important correlate to clinical disability and disease duration in multiple sclerosis. The objective of this study was to link brain atrophy visualized by neuroimaging to its underlying neuropathology using the MS model, experimental autoimmune encephalomyelitis (EAE). Volumetric changes in brains of EAE mice, as well as matched healthy normal controls, were quantified by collecting post-mortem high-resolution T2-weighted magnetic resonance microscopy and actively stained magnetic resonance histology images. Anatomical delineations demonstrated a significant decrease in the volume of the whole cerebellum, cerebellar cortex, and molecular layer of the cerebellar cortex in EAE as compared to normal controls. The pro-apoptotic marker caspase-3 was detected in Purkinje cells and a significant decrease in Purkinje cell number was found in EAE. Cross modality and temporal correlations revealed a significant association between Purkinje cell loss on neuropathology and atrophy of the molecular layer of the cerebellar cortex by neuroimaging. These results demonstrate the power of using combined population atlasing and neuropathology approaches to discern novel insights underlying gray matter atrophy in animal models of neurodegenerative disease. Published by Elsevier Inc.