Resolution of parallel and antiparallel oligonucleotide triple helices formation and melting processes by multivariate curve resolution
Resolution of parallel and antiparallel oligonucleotide triple helices formation and melting processes by multivariate curve resolution
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DOI：
10.1080/07391102.2003.10506922
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发表时间：
2003-10-01
影响因子：
4.4
通讯作者：
Gargallo, R
中科院分区：
文献类型：
--
作者：
Jaumot, J;Aviñó, A;Gargallo, R
描述了一种程序，用于作为pH和温度的函数的寡核苷酸三链体的浓度曲线的完整分辨率。通过对沿着酸碱滴定和单链、发夹及其混合物的融化实验记录的紫外吸收光谱进行多变量曲线分辨率分析，成功估计了三链体存在的pH和温度范围以及参与酸碱和构象平衡的所有物质的相对浓度。成功地估计了形成常数对pH的依赖性。合成了发夹h26（5 ′-GAAGGAGGAGA-TTTT-TCTCCTCCTTC-3 ′）和单链寡核苷酸s11 CT（5 ′-CTTCCTCCTCT-3 ′）、s11 AG（5 ′-AGAGGAGGAAG-3 ′）和s11 TG（5 ′-TGTGGTGGTTG-3 ′），并对其质子化和构象平衡进行了详细研究。该程序被证明是特别有用的三链体与低的减色后形成的研究。
A procedure is described for the complete resolution of concentration profiles of oligonucleotide triplexes as a function of pH and temperature. The pH and temperature ranges at which triplexes are present and the relative concentrations of all the species involved in acid-base and conformational equilibria are successfully estimated from Multivariate Curve Resolution analysis of UV absorbance spectra recorded along acid-base titrations and melting experiments of single stranded, hairpin and their mixtures. The dependence of formation constants upon pH was successfully estimated. The hairpin h26 (5'-GAAGGAGGAGA-TTTT-TCTCCTCCTTC-3'), and the single stranded oligonucleotides s11CT (5'-CTTCCTCCTCT-3'), s11AG (5'-AGAGGAGGAAG-3') and s11TG (5'-TGTGGTGGTTG-3') were synthesized and their protonation and conformational equilibria were studied in detail. The procedure was shown to be especially useful for the study of triplexes with a low hypochromism upon formation.