Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology
Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology
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DOI：
10.3791/51294
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发表时间：
2014-06-01
影响因子：
1.2
通讯作者：
Dai, Guohao
中科院分区：
文献类型：
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作者：
Cui, Xiaofeng;Gao, Guifang;Dai, Guohao
生物打印以热敏喷墨打印为基础，是组织工程和再生医学领域最具吸引力的技术之一。通过数字控制细胞、支架和生长因子可以快速精确地沉积到所需的二维（2D）和三维（3D）位置。因此，该技术是一种理想的方法来制造组织模仿其本身的解剖结构。为了使软骨具有天然的带状组织、细胞外基质组成（ECM）和机械性能，我们开发了一个生物打印平台，使用商用喷墨打印机，同时具有光聚合能力，可用于3D软骨组织工程。将悬浮在聚乙二醇二丙烯酸酯（PEGDA）中的人软骨细胞通过逐层组装打印出来，用于3D构建新软骨。打印的细胞被固定在其原始沉积位置，由周围支架支撑，同时进行光聚合。打印组织的力学性能与天然软骨相似。与需要更长时间紫外线照射的传统组织制造相比，同时光聚合的打印细胞的活力明显更高。打印的新软骨表现出良好的糖胺聚糖（GAG）和II型胶原生成，这与基因表达一致。因此，该平台是解剖组织工程中精确的细胞分布和排列的理想平台。
Bioprinting, which is based on thermal inkjet printing, is one of the most attractive enabling technologies in the field of tissue engineering and regenerative medicine. With digital control cells, scaffolds, and growth factors can be precisely deposited to the desired two-dimensional (2D) and three-dimensional (3D) locations rapidly. Therefore, this technology is an ideal approach to fabricate tissues mimicking their native anatomic structures. In order to engineer cartilage with native zonal organization, extracellular matrix composition (ECM), and mechanical properties, we developed a bioprinting platform using a commercial inkjet printer with simultaneous photopolymerization capable for 3D cartilage tissue engineering. Human chondrocytes suspended in poly(ethylene glycol) diacrylate (PEGDA) were printed for 3D neocartilage construction via layer-by-layer assembly. The printed cells were fixed at their original deposited positions, supported by the surrounding scaffold in simultaneous photopolymerization. The mechanical properties of the printed tissue were similar to the native cartilage. Compared to conventional tissue fabrication, which requires longer UV exposure, the viability of the printed cells with simultaneous photopolymerization was significantly higher. Printed neocartilage demonstrated excellent glycosaminoglycan (GAG) and collagen type II production, which was consistent with gene expression. Therefore, this platform is ideal for accurate cell distribution and arrangement for anatomic tissue engineering.