CLEAVAGE AT A V(D)J RECOMBINATION SIGNAL REQUIRES ONLY RAG1 AND RAG2 PROTEINS AND OCCURS IN 2 STEPS
CLEAVAGE AT A V(D)J RECOMBINATION SIGNAL REQUIRES ONLY RAG1 AND RAG2 PROTEINS AND OCCURS IN 2 STEPS
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DOI：
10.1016/0092-8674(95)90116-7
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发表时间：
1995-11-03
期刊：
影响因子：
64.5
通讯作者：
OETTINGER, MA
中科院分区：
文献类型：
--
作者：
MCBLANE, JF;VANGENT, DC;OETTINGER, MA
在重组信号序列处形成双链断裂是V（D）J重组的早期步骤。在这里，我们表明，纯化的RAG 1和RAGS蛋白足以进行这一反应。裂解反应可以分为两个不同的步骤。首先，在信号序列的5'端引入切口，然后另一条链断裂，在编码端产生发夹结构和钝的5'-磷酸化信号端。发夹是作为切割机制的直接结果而产生的。切口和发夹形成各自需要信号序列和两种RAG蛋白的存在。
Formation of double-strand breaks at recombination signal sequences is an early step in V(D)J recombination. Here we show that purified RAG1 and RAGS proteins are sufficient to carry out this reaction. The cleavage reaction can be divided into two distinct steps. First, a nick is introduced at the 5' end of the signal sequence, The other strand is then broken, resulting in a hairpin structure at the coding end and a blunt, 5'-phosphorylated signal end. The hairpin is made as a direct consequence of the cleavage mechanism. Nicking and hairpin formation each require the presence of a signal sequence and both RAG proteins.