Increased ceruloplasmin expression caused by infiltrated leukocytes, activated microglia, and astrocytes in injured female rat spinal cords.
Increased ceruloplasmin expression caused by infiltrated leukocytes, activated microglia, and astrocytes in injured female rat spinal cords.
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受损雌性大鼠脊髓中浸润的白细胞、活化的小胶质细胞和星形胶质细胞引起铜蓝蛋白表达增加。
DOI：
10.1002/jnr.24221
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Lü He Zuo
中科院分区：
文献类型：
--
作者：
Wu Yan;Shen Lin;Wang Rui;Tang Jie;Ding Shu Qin;Wang Sai Nan;Guo Xue Yan;Hu Jian Guo;Lü He Zuo
铜蓝蛋白（Cp）是一种含有六个铜原子的酶，在铁稳态和抗氧化防御中起重要作用。脊髓损伤（SCI）后，局部微环境中的细胞成分非常复杂，包括驻留细胞的功能变化和白细胞的浸润。已经证实，在小鼠SCI后，Cp主要在星形胶质细胞中升高，在巨噬细胞中升高的程度较小。然而，它在其他细胞类型中的表达仍然不是很清楚。在这份手稿中，我们通过在雌性Sprague-Dawley大鼠模型中检查该系统并将调查范围扩大到包括其他细胞类型来提供这些发现的合理扩展。定量逆转录聚合酶链反应和蛋白质印迹分析显示，在SCI组织匀浆Cp mRNA和蛋白质是相当一致的，与以前的出版物。然而，我们观察到Cp不仅在GFAP+星形胶质细胞中表达（与先前的报道一致），而且在CD 11b+小胶质细胞、CN+少突胶质细胞、NeuN+神经元、CD 45+白细胞和CD 68+活化的小胶质细胞/巨噬细胞中也表达。定量分析表明，浸润的白细胞，活化的小胶质细胞/巨噬细胞和星形胶质细胞应该是增加Cp的主要来源。
Ceruloplasmin (Cp), an enzyme containing six copper atoms, has important roles in iron homeostasis and antioxidant defense. After spinal cord injury (SCI), the cellular components in the local microenvironment are very complex and include functional changes of resident cells and the infiltration of leukocytes. It has been confirmed that Cp is elevated primarily in astrocytes and to a lesser extent in macrophages following SCI in mice. However, its expression in other cell types is still not very clear. In this manuscript, we provide a sensible extension of these findings by examining this system within a female Sprague–Dawley rat model and expanding the scope of inquiry to include additional cell types. Quantitative reverse transcription polymerase chain reaction and Western blot analysis revealed that the Cp mRNA and protein in SCI tissue homogenates were quite consistent with prior publications. However, we observed that Cp was expressed not only in GFAP+astrocytes (consistent with prior reports) but also in CD11b+microglia, CNPase+oligodendrocytes, NeuN+neurons, CD45+leukocytes, and CD68+activated microglia/macrophages. Quantitative analysis proved that infiltrated leukocytes, activated microglia/macrophages, and astrocytes should be the major sources of increased Cp.