Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions
Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions
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DOI：
10.1016/j.bjp.2014.07.019
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发表时间：
2014-08-01
期刊：
影响因子：
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通讯作者：
Pinto, Ernani
中科院分区：
文献类型：
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作者：
Bortoli, Stella;Oliveira-Silva, Diogo;Pinto, Ernani
蓝藻是一种原核生物和光合生物，可以产生多种具有不同性质的生物活性化合物；包括各种有毒化合物，也被称为蓝藻毒素。在这项工作中，我们描述了从巴西圣保罗州的两个水库分离出的七个蓝藻菌株。在10株分离菌株中的3株中检测到7种不同的微囊藻毒素化学变异体（MC-RR、MC-LR、MC-YR、MC-LF、MC-LW和2种去甲基化变异体dm-MC-RR和dm-MC-LR）。选择一株特殊的铜绿微囊藻菌株（LTPNA 02），通过细胞计数和7种不同营养方案下的毒素产量来评估其生长情况。在对数生长期，只有3个实验观察到不同的生长行为（p < 0.05）。总生长分析发现4个试验与对照组差异显著（p < 0.01）。采用液相色谱-串联质谱法对3种微囊藻毒素（MC-RR、MC-LR和MC-YR）进行定量分析。实验结束时，比较ASM-1 （N:P = 1）、MLA和BG-11 （N:P = 10）培养基中细胞的生长情况，毒素含量不变。在所有其他实验中，在Bold 3N培养基中生长的细胞在指数生长阶段的微囊藻毒素产量最低。在BG-11（N:P = 100）培养基中微囊藻毒素含量最高。(C) 2014年巴西农学会。由爱思唯尔编辑有限公司出版。版权所有。
Cyanobacteria are prokaryotic and photosynthetic organisms, which can produce a wide range of bioactive compounds with different properties; including a variety of toxic compounds, also known as cyanotoxins. In this work, we describe the isolation of seven cyanobacterial strains from two reservoirs in Sao Paulo State, Brazil. Seven different chemical variants of microcystins (MC-RR, MC-LR, MC-YR, MC-LF, MC-LW, and two demethylated variants, dm-MC-RR and dm-MC-LR) were detected in three of the ten isolated strains. One particular Microcystis aeruginosa strain (LTPNA 02) was chosen to evaluate its growth by cell count, and its toxin production under seven different nutritional regimes. We observed different growth behaviors in the logarithmic growth period for only three experiments (p < 0.05). The total growth analysis identified four experiments as different from the control (p < 0.01). Three microcystin variants (MC-RR, MC-LR and MC-YR) were quantified by liquid chromatography-tandem mass spectrometry. At the experimental end, the toxin content was unchanged when comparing cell growth in ASM-1 (N:P = 1), MLA and BG-11 (N:P = 10) medium. In all other experiments, the lowest microcystin production was observed from cells grown in Bold 3N medium during the exponential growth phase. The highest microcystin content was observed in cultures using BG-11(N:P = 100) medium. (C) 2014 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved.