Small intestinal microbiota composition and the prognosis of infants with ileostomy resulting from distinct primary diseases
Small intestinal microbiota composition and the prognosis of infants with ileostomy resulting from distinct primary diseases
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不同原发性疾病所致回肠造口婴儿的小肠微生物群组成与预后
DOI：
10.1186/s12876-020-01366-0
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发表时间：
2020-07-13
影响因子：
2.4
通讯作者：
Zhang, Rong
中科院分区：
文献类型：
--
作者：
Qian, Tian;Zhu, Haitao;Zhang, Rong
由于各种病因，对小肠造口术婴儿的微生物群组成的研究有限。在这里，我们的特点是肠道微生物群的新生儿回肠造口术所造成的不同的原发性疾病。方法15例坏死性小肠结肠炎，8例胎粪性腹膜炎，7例先天性巨结肠。研究了由这些疾病引起的回肠造口术婴儿的小肠微生物群组成。结果新生儿回肠造口液中微生物多样性普遍较低，以变形菌门和厚壁菌门成员为主。在属级水平上，以克雷伯氏菌、志贺菌、链球菌、狭义梭菌、肠球菌和乳杆菌属最多，链球菌和韦荣氏菌与碳水化合物代谢和免疫有关，母乳喂养可增加这些潜在有益菌的比例。母乳喂养组双歧杆菌比例高于非母乳喂养组，结肠炎和脓毒症发生率低于非母乳喂养组。与非母乳喂养组相比，母乳喂养组双歧杆菌比例增加，结肠炎和脓毒症发生率降低，但差异无统计学意义。观察到母乳喂养组的体重增加高于非母乳喂养组。结论回肠造口后克雷伯氏菌、志贺菌数量增多，链球菌、韦荣氏菌、粪杆菌数量减少，提示回肠造口后肠道菌群处于不健康状态。然而，链球菌、粪杆菌和韦荣氏球菌经常出现，这表明这些细菌的扩增可能有助于术后免疫系统的发育。
Background Studies of microbiota composition of infants with small intestinal ostomy due to various etiologies are limited. Here, we characterized the intestinal microbiota of neonates with ileostomy resulting from distinct primary diseases. Methods Fifteen patients with necrotizing enterocolitis, eight patients with meconium peritonitis, and seven patients with Hirschsprung's disease were included in the study. The small intestinal microbiota composition in infants with ileostomy caused by these diseases was studied. Results Microbial diversity in neonatal ileostomy fluid was generally low, and was dominated by members of the Proteobacteria and Firmicutes phyla. At the genus level, the most abundant wereKlebsiella,Escherichia-Shigella,Streptococcus,Clostridiumsensu stricto 1,Enterococcus, andLactobacillus.StreptococcusandVeillonellaare related to carbohydrate metabolism and immunity, and breastfeeding can increase the proportion of these potentially beneficial bacteria. The proportion ofBifidobacteriumin the breastfeeding group was higher than that in the non-breastfeeding group, and incidence of colitis and sepsis was reduced in the breastfeeding group. The proportion ofBifidobacteriumincreased and incidence of colitis and sepsis decreased in the breastfeeding group compared with the non- breastfeeding group, but there was no significant difference. The increase in body weight in the breastfeeding group was observed to be higher than in the non-breastfeeding group. Conclusions ExcessiveKlebsiellaandEscherichia-Shigellaand low abundance ofStreptococcus,VeillonellaandFaecalibacteriumsuggests that the small intestinal microbiota is in an unhealthy state after ileostomy. However,Streptococcus,Faecalibacterium, andVeillonellaspecies were frequently present, suggesting that expansion of these bacteria might assist the development of the immune system after surgery.