Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products.
Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products.
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DOI：
10.1155/2017/1352948
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Wagiran A
中科院分区：
文献类型：
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作者：
Mohammed Abubakar B;Mohd Salleh F;Shamsir Omar MS;Wagiran A
在过去的二十年中，由于其声称的健康益处，草药产品（HMP）的全球使用量大幅增加。这导致其需求增加，因此也导致了大规模的掺假。这是由于大多数传统方法无法识别工艺产品形式中密切相关的物种。因此，迫切需要简单和快速的鉴定方法导致了一种新技术的发现。DNA条形码是使用来自标准基因组的短DNA序列进行物种鉴定的过程。该技术可靠，不受气候，年龄或植物部位等外部因素的影响。除了其他因素之外，分离高质量DNA的困难是使用DNA条形码鉴定HMP时遇到的挑战之一。这些局限性表明，单独使用DNA条形码可能无法有效地鉴定HMP。因此，DNA条形码技术与色谱指纹图谱技术相结合，将为有效评价HMP的真实性和质量一致性提供一种有效的解决方案。关于HMP主要成分的详细和质量信息将有助于确定其功效和安全性，因为这些对于质量控制非常重要。
In the last two decades, there has been a tremendous increase in the global use of herbal medicinal products (HMPs) due to their claimed health benefits. This has led to increase in their demand and consequently, also, resulted in massive adulteration. This is due to the fact that most of the traditional methods cannot identify closely related species in a process product form. Therefore the urgent need for simple and rapid identification methods resulted in the discovery of a novel technique. DNA barcoding is a process that uses short DNA sequence from the standard genome for species identification. This technique is reliable and is not affected by external factors such as climates, age, or plant part. The difficulties in isolation of DNA of high quality in addition to other factors are among the challenges encountered using the DNA barcoding in the authentication of HMP. These limitations indicated that using DNA barcoding alone may ineffectively authenticate the HMP. Therefore, the combination of DNA barcoding with chromatographic fingerprint, a popular and generally accepted technique for the assessment and quality control of HMP, will offer an efficient solution to effectively evaluate the authenticity and quality consistency of HMP. Detailed and quality information about the main composition of the HMPs will help to ascertain their efficacy and safety as these are very important for quality control.