Localization of prostaglandin D2 binding protein and NADP-linked 15-hydroxyprostaglandin D2 dehydrogenase in the Purkinje cells of miniature pig cerebellum.
Localization of prostaglandin D2 binding protein and NADP-linked 15-hydroxyprostaglandin D2 dehydrogenase in the Purkinje cells of miniature pig cerebellum.
复制标题
前列腺素 D2 结合蛋白和 NADP 连接的 15-羟基前列腺素 D2 脱氢酶在小型猪小脑浦肯野细胞中的定位。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
O. Hayaishi
中科院分区：
文献类型：
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作者：
Y. Watanabe;A. Yamashita;H. Tokumoto;O. Hayaishi
通过计算机图像处理和颜色编码辅助的放射自显影技术，可视化前列腺素D2特异性的高亲和力结合位点。在小型猪小脑中，在皮质中观察到高密度的结合，主要在浦肯野细胞层。NADP连接的15-羟基前列腺素D2脱氢酶的细胞定位的免疫组织化学研究进行了相同的小脑，并揭示了这种酶的存在下，也在浦肯野细胞。这些发现意味着前列腺素D2与浦肯野细胞结合，并且在其作用后，通过特异性降解酶原位降解。
High-affinity binding sites specific for prostaglandin D2 were visualized by the autoradiographical technique assisted by computerized image processing and color coding. In the cerebellum of miniature pig, high density of the binding was observed in the cortex, mainly in the Purkinje cell layer. Immunohistochemical studies on the cellular localization of NADP-linked 15-hydroxyprostaglandin D2 dehydrogenase were performed with the same cerebellum and revealed the presence of this enzyme also in the Purkinje cells. These findings imply that prostaglandin D2 binds to the Purkinje cells and, after its action, is degraded in situ by the specific degradative enzyme.