Genome-wide misexpression of X-linked versus autosomal genes associated with hybrid male sterility
Genome-wide misexpression of X-linked versus autosomal genes associated with hybrid male sterility
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DOI：
10.1101/gr.076620.108
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发表时间：
2010-08-01
期刊：
影响因子：
7
通讯作者：
Wu, Chung-I
中科院分区：
文献类型：
--
作者：
Lu, Xuemei;Shapiro, Joshua A.;Wu, Chung-I
交配后的生殖隔离通常表现为杂种雄性不育，其中X连锁基因被过度代表（所谓的大X效应）。相反，X连锁基因在睾丸表达基因中的代表性明显不足。这种表面上的矛盾可能与X：常染色体不平衡假说有关，在该假说中，X连锁效应主要是通过常染色体基因的错误表达介导的。在这项研究中，我们比较了基因表达的可育和不育男性之间的杂交果蝇物种。这些杂交雄性的差异仅在于X染色体上包含杂交不育的Ods位点同源盒（OdsH）（也称为Odysseus）基因座的一小部分。在睾丸中表达的基因中，常染色体基因确实比X连锁基因在OdsH的绝育作用下更容易被错误表达。由于这种X：常染色体相互作用的机制只与精子发生有关，因此X：常染色体不平衡与高杂交雄性不育率之间的联系似乎是合理的。
Postmating reproductive isolation is often manifested as hybrid male sterility, for which X-linked genes are over-represented (the so-called large X effect). In contrast, X-linked genes are significantly under-represented among testis-expressing genes. This seeming contradiction may be germane to the X:autosome imbalance hypothesis on hybrid sterility, in which the X-linked effect is mediated mainly through the misexpression of autosomal genes. In this study, we compared gene expression in fertile and sterile males in the hybrids between two Drosophila species. These hybrid males differ only in a small region of the X chromosome containing the Ods-site homeobox (OdsH)(also known as Odysseus) locus of hybrid sterility. Of genes expressed in the testis, autosomal genes were, indeed, more likely to be misexpressed than X-linked genes under the sterilizing action of OdsH. Since this mechanism of X:autosome interaction is only associated with spermatogenesis, a connection between X:autosome imbalance and the high rate of hybrid male sterility seems plausible.