Coupling Microdialysis Sampling to Microchip Electrophoresis in a Reversibly Sealed Device.
Coupling Microdialysis Sampling to Microchip Electrophoresis in a Reversibly Sealed Device.
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DOI：
10.1016/j.jala.2007.04.008
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发表时间：
2007-10-01
期刊：
影响因子：
--
通讯作者：
Martin, R Scott
中科院分区：
文献类型：
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作者：
Mecker, Laura C;Martin, R Scott
在本文中，我们描述了一个可逆密封的微芯片装置的制造和表征，该装置用于耦合微透析取样和微芯片电泳。从重复使用的角度来看，将微透析采样和微芯片电泳接口在可可逆密封的设备中是有利的。来自微透析探头的市售管道直接插入芯片，探头的流量通过集成气动阀连接到设备的电泳部分。荧光检测用于表征基于聚二甲基硅氧烷的装置在注射重现性方面。结果发现，整个系统（微透析探针和微芯片装置）的浓度响应滞后时间为170秒。微透析取样后，用萘-2,3-二甲醛/氰化物衍生的氨基酸也得到了电泳分离。
In this paper, we describe the fabrication and characterization of a reversibly sealed microchip device that is used to couple microdialysis sampling to microchip electrophoresis. The ability to interface microdialysis sampling and microchip electrophoresis in a device that is amenable to reversible sealing is advantageous from a repeated use standpoint. Commercially available tubing coming from the microdialysis probe is directly inserted into the chip and flow from the probe is interfaced to the electrophoresis portion of the device through integrated pneumatic valves. Fluorescence detection was used to characterize the poly(dimethylsiloxane)-based device in terms of injection reproducibility. It was found that the entire system (microdialysis probe and microchip device) has a concentration response lag time of 170 sec. Microdialysis sampling followed by an electrophoretic separation of amino acids derivatized with naphthalene-2,3-dicarboxaldehyde/cyanide was also demonstrated.