Development of a side-view chamber for studying cell-surface adhesion under flow conditions
Development of a side-view chamber for studying cell-surface adhesion under flow conditions
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DOI：
10.1007/bf02684196
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发表时间：
1997-05-01
影响因子：
3.8
通讯作者：
Dong, C
中科院分区：
文献类型：
--
作者：
Cao, J;Usami, S;Dong, C
观察显微标本在研究细胞与血管壁的相互作用时通常是有用的。我们已经开发了一种体外侧视流动室，允许从动态流动条件下细胞与各种粘合剂表面接触的一侧进行观察。这种侧视流动室由两个称为微载玻片的精密矩形玻璃管组成。将较小的载玻片插入较大的载玻片中，以创建具有平坦表面的流动通道，在该流动通道上可以生长培养的血管内皮或可以涂覆纯化的粘附分子。沿流动通道沿着使用两个具有45度镀铬表面的光学棱镜，以产生光照明和观察路径。细胞-基质接触的侧视图图像可以使用光学显微镜获得。这种设计使我们不仅可以测量流动对细胞表面粘附强度的影响，而且可以密切观察剪切流中细胞变形和与各种表面的粘附接触。此外，该室可以容易地用作常规的俯视流动通道，类似于在许多领域中使用的平行板流动室。这种侧视流动室的发展可以有利于细胞研究中需要边缘视图的各种体外应用，特别是对于在流动条件下与培养的血管内皮或含有单一类型粘附分子的表面的各种细胞相互作用。
Observing microscopic specimens is often useful in studies of cellular interaction with a vascular wall. We have developed an in vitro side-view flow chamber that permits observations from the side of the cell's contact with various adhesive surfaces under dynamic flow conditions. This side-view flow chamber consists of two precision rectangular glass tubes called microslides. A smaller microslide is inserted into a larger one to create a flow channel with a flat surface on which either cultured vascular endothelium can be grown or purified adhesion molecules can be coated. Two optical prisms with a 45 degrees chromium-coated surface are used along the flow channel to generate light illumination and observation pathways. The side-view images of cell-substrate contact can be obtained using a light microscope. This design allows us not only to measure the effects of flow on cell-surface adhesion strength, but also to have close observation of cell deformation and adhesive contact to various surfaces in shear flow. In addition, this chamber can readily serve for a conventional top-view flow channel, similar to the parallel-plate flow chambers used in many areas. The development of such a side-view flow chamber can be beneficial to various in vitro applications in cellular studies that require an edge view, especially for various cell interactions with cultured vascular endothelium or surfaces containing single-type adhesive molecules under flow conditions.