Expression pattern of PD-L1 and PD-L2 in classical Hodgkin lymphoma, primary mediastinal large B-cell lymphoma, and gray zone lymphoma
Expression pattern of PD-L1 and PD-L2 in classical Hodgkin lymphoma, primary mediastinal large B-cell lymphoma, and gray zone lymphoma
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DOI：
10.1111/ejh.13033
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发表时间：
2018-05-01
影响因子：
3.1
通讯作者：
Kobayashi, Yukio
中科院分区：
文献类型：
--
作者：
Tanaka, Yosuke;Maeshima, Akiko Miyagi;Kobayashi, Yukio
目的：我们旨在从程序性细胞死亡1和程序性死亡配体（PDL）网络的畸变角度，探讨经典霍奇金淋巴瘤（cHL）、原发性纵隔大B细胞淋巴瘤（PMBL）和具有cHL和PMBL中间特征的灰区淋巴瘤（GZL）之间的关系。方法：我们探讨了67例中PDLs和染色体异常的表达水平：34例使用免疫组织化学分析和荧光原位杂交 (FISH)，其中 21 例为 cHL，20 例为 PMBL，13 例为 GZL。结果：21 例 cHL (62%)、3 例 PMBL (15%) 和 6 例 GZL (46%) 病例显示超过 70% 的肿瘤细胞中 PD-L1 抗体染色。 2 例 cHL (6%)、10 例 PMBL (50%) 和 3 例 GZL (23%) 病例未被 PD-L1 抗体染色。 40岁以上的患者在cHL中表现出更频繁的PD-L1表达。 PMBL 中 PD-L2 抗体染色的肿瘤比例增加。使用 PD-L1/PD-L2 探针进行 FISH 分析，检测到 cHL 中有 5 个扩增、1 个增益和 7 个多体性病例，GZL 中有 1 个扩增和 1 个多体性病例，1 个 PMBL 病例有扩增。结论：我们发现 cHL 中 PD-L1 染色增加，PMBL 中 PD-L2 染色增加。 GZL 的模式与 cHL 类似。
Objectives: We aimed at investigating the relationship between classical Hodgkin lymphoma (cHL), primary mediastinal large B-cell lymphoma (PMBL), and gray zone lymphoma (GZL) with intermediate characteristics between cHL and PMBL, from the perspective of the aberration in programed cell death 1 and the programed death ligands (PDLs) network.Methods: We explored the expression levels of PDLs and chromosomal anomalies in 67 cases: 34 cases with cHL, 20 with PMBL, and 13 with GZL, using immunohistochemical analyses and Fluorescence In Situ Hybridization (FISH).Results: Twenty-one cHL (62%), 3 PMBL (15%), and 6 GZL (46%) cases showed staining to PD-L1 antibodies in more than 70% of tumor cells. Two cHL (6%), 10 PMBL (50%), and 3 GZL (23%) cases were not stained by PD-L1 antibodies. Patients over 40 years old manifest more frequent expression of PD-L1 in cHL. Proportion of tumors stained by PD-L2 antibody was increased in PMBL. FISH analyses with a PD-L1/PD-L2 probe detected 5 amplification, 1 gain, and 7 polysomy cases in cHL, 1 amplification and 1 polysomy case in GZL, and amplification in 1 PMBL case.Conclusion: We identified increased staining of PD-L1 in cHL and that of PD-L2 in PMBL. GZL had a pattern similar to that of cHL.