A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes.
A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes.
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DOI：
10.1016/j.bone.2003.12.016
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发表时间：
2004-04
期刊：
影响因子：
4.1
通讯作者：
K. Yagami;Yohei Uyama;Y. Yoshizawa;S. Kakuta;A. Yamaguchi;M. Nagumo
中科院分区：
文献类型：
--
作者：
K. Yagami;Yohei Uyama;Y. Yoshizawa;S. Kakuta;A. Yamaguchi;M. Nagumo
我们研究了从人软骨性骨肉瘤中分离的克隆细胞系（USAC）向各种间充质细胞系分化的潜力。USAC细胞产生II型和X型胶原和蛋白多糖，表明软骨细胞分化。USAC细胞的I型胶原和骨钙素的产生证明了成骨细胞的特性。它们还分化成脂肪细胞，在其细胞质中产生大量脂滴。重组人骨形态发生蛋白-2（rhBMP-2）增加蛋白多糖、II型和X型胶原和骨钙素的产生。这表明rhBMP-2促进USAC细胞中成软骨细胞和成骨细胞的分化。rhBMP-2抑制脂肪细胞分化。将USAC细胞与rhBMP-2一起移植到使用扩散室的无胸腺小鼠的腹膜腔中，在扩散室中比不使用rhBMP-2产生的细胞更有效地产生软骨和骨。尽管USAC细胞在有或没有rhBMP-2的情况下移植后在扩散室中也产生脂肪组织，但rhBMP-2处理减少了脂肪组织的量。这些结果表明，USAC是一个合适的模型，以探讨参与人间充质细胞分化的调控机制。
We investigated the differentiation potential into various mesenchymal cell lineages of the clonal cell line (USAC) that was isolated from a human chondrogenic osteosarcoma. USAC cells produced types II and X collagens and proteoglycan, indicating chondrocyte differentiation. Production of type I collagen and osteocalcin by USAC cells demonstrated osteoblastic properties. They also differentiated into adipocytes generating numerous lipid droplets in their cytoplasm. Recombinant human bone morphogenetic protein-2 (rhBMP-2) increased production of proteoglycan, types II and X collagens and osteocalcin. This indicates that rhBMP-2 promotes both chondroblastic and osteoblastic differentiation in USAC cells. rhBMP-2 inhibited adipocyte differentiation. USAC cells transplanted with rhBMP-2 into the peritoneal cavities of athymic mice using diffusion chambers generated cartilage and bone more effectively in the diffusion chambers than those produced without rhBMP-2. Although USAC cells also produced adipose tissue in the diffusion chambers following transplantation with or without rhBMP-2, rhBMP-2 treatment reduced the amounts of adipose tissue. These results demonstrate that USAC is a suitable model to explore regulatory mechanisms involved in human mesenchymal cell differentiation.