Inheritance, fitness cost and mechanism of resistance to tebufenozide in Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae)
Inheritance, fitness cost and mechanism of resistance to tebufenozide in Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae)
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DOI：
10.1002/ps.1785
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发表时间：
2009-09-01
影响因子：
4.1
通讯作者：
Shen, Jinliang
中科院分区：
文献类型：
--
作者：
Jia, Biantao;Liu, Yongjie;Shen, Jinliang
背景：虫酰肼是防治甜菜夜蛾的关键杀虫剂。为了评估抗性进化的风险并制定更好的抗性管理策略，在实验室中用虫酰肼选择田间收集的种群。在此基础上，对虫酰肼抗性的遗传和适合度代价进行了研究。结果：用虫酰肼选择61代后，甜菜夜蛾产生了抗性。甜菜对该化学品产生了92倍的抗性。正反交后代的显性度分别为-0.2698和-0.2785。抗性品系的相对适合度为0.71，幼虫存活率、蛹重、化蛹率和每雌产卵率显著降低，幼虫和蛹历期延长。PBO显著提高了虫酰肼（SR = 2）对抗性昆虫的毒力，DEM和DEF对虫酰肼也表现出增效作用。甜菜为常染色体不完全隐性遗传，受多基因控制。抗性的发展可能会使抗性种群的适应性大大降低。混合功能氧化酶可能在虫酰肼抗性中起重要作用。exigua.本研究为进一步了解虫酰肼的抗药性和制定抗性治理策略提供了有价值的信息。(C)2009年化学工业协会
BACKGROUND: Tebufenozide has been used as a key insecticide for controlling beet armyworm, Spodoptero exigua (Hubner). To evaluate the risk of resistance evolution and to develop a better resistance management strategy, a field-collected population was selected with tebufenozide in the laboratory. Thereafter, the inheritance and fitness cost of tebufenozide resistance in S. exigua were investigated.RESULTS: After being selected with tebufenozide for 61 generations, S. exigua developed a 92-fold resistance to the chemical. The degrees of dominance for the reciprocal cross progeny were -0.2698 and -0.2785. The resistant strain had a relative fitness of 0.71, with substantially lower rates of larval survival, pupal weight, pupation and oviposition per female, and prolonged larval and pupal duration. PBO significantly increased the toxicity of tebufenozide (SR = 2) against resistant insects, and DEM and DEF also showed synergism with tebufenozide.CONCLUSION: Tebufenozide resistance in S. exigua was inherited as autosomal, incompletely recessive and controlled by more than one gene. Development of the resistance may cost significant fitness for the resistant population. Mixed-function oxidases might play an important role in tebufenozide resistance in S. exigua. This study provided valuable information for further understanding tebufenozide resistance and for facilitating the development of resistance management strategies. (C) 2009 Society of Chemical Industry