PHOSPHORYLATION DETERMINES 2 DISTINCT SPECIES OF TAU IN THE CENTRAL-NERVOUS-SYSTEM
PHOSPHORYLATION DETERMINES 2 DISTINCT SPECIES OF TAU IN THE CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1002/cm.970080303
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发表时间：
1987-01-01
影响因子：
--
通讯作者：
BINDER, LI
中科院分区：
文献类型：
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作者：
PAPASOZOMENOS, SC;BINDER, LI
单克隆抗体 Tau-1 以前用于将 tau 蛋白定位于大脑轴突区室，在磷酸酶处理组织后，已重新用于光和电子显微镜免疫组织化学。我们在此报告，中枢神经系统中的大量 tau 在 Tau-1 表位处或附近原位磷酸化，从而阻止 Tau-1 抗体的结合。然而，在去除这个/这些磷酸基团后，在神经元的体树突区室以及轴突中观察到 Tau-1。此外，在星形胶质细胞和神经周围神经胶质细胞中也观察到强烈的染色。这种免疫反应性存在于微管的长度和核糖体（多核糖体）上。用磷酸酶处理整个大脑皮层提取物的免疫印迹证实了免疫组织化学结果，注意到 Tau-1 与印迹 tau 区域的结合增加了 50-65%。此外，这些实验中还使用了一种新型单克隆抗体 Tau-2。该抗体仅与 tau 蛋白结合，并沿着轴突、胞体、树突和星形胶质细胞中的微管以及核糖体（多核糖体）定位，无需磷酸酶预处理。
The monoclonal antibody, Tau-1, which had previously been used to localize tau to the axonal compartment in brain has been reutilized for light and electron microscopic immunohistochemistry following phosphatase treatment of tissue. We report here that a signficiant quantity of tau in the central nervous system is phosphorylated in situ at or near the Tau-1 epitope, preventing the binding of the Tau-1 antibody. Upon removal of this/these phosphate group(s), however, Tau-1 was observed in the somatodendritic compartment of neurons as well as in axons. Furthermore, intense staining was also observed in astrocytes and in perineuronal glial cells. This immunoreactivity was present along the lengths of microtubules and on ribosomes (polysomes). Treatment of immunoblots of extracts of whole cerebral cortex with phosphatase confirmed the immunohistochemical results in that a 50-65% increase in Tau-1 binding to the tau region of the blot was noted. Moreover, a novel monoclonal antibody, Tau-2, was also used in these experiments. This antibody binds only to tau and localizes along microtubules in axons, somata, dendrites, and astrocytes and on ribosomes (polysomes) without phosphatase pretreatment.