Downregulation of OCLN and GAS1 in clear cell renal cell carcinoma
Downregulation of OCLN and GAS1 in clear cell renal cell carcinoma
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DOI：
10.3892/or.2017.5414
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发表时间：
2017-03-01
期刊：
影响因子：
4.2
通讯作者：
Rahal, Paula
中科院分区：
文献类型：
--
作者：
Conceicao, Andre Luis Giacometti;Da Silva, Camila Tainah;Rahal, Paula
透明细胞肾细胞癌（ccRCC）是肾癌最常见的组织学亚型。这种癌的组织学特征是存在透明和丰富的胞浆。在本研究中，我们试图鉴定在ccRCC中差异表达的基因，并建立这种癌症的分子谱。我们选择了文献中描述的与细胞分化和增殖相关的基因。我们分别通过定量PCR（qPCR）和免疫组织化学分析了基因和蛋白质表达，并研究了调控其在ccRCC样品和细胞系中表达的可能表观遗传机制。封闭蛋白（OCLN）和生长停滞特异性1（GAS 1）基因在ccRCC中表达不足，我们报告miR-122和miR-34 a分别可以调节它们在这种癌症中的表达。此外，我们通过qPCR和免疫组织化学显示溶质载体家族2成员1（SLC 2A 1）在ccRCC中显著过表达。本研究中鉴定的基因组进一步加深了我们对ccRCC的分子基础和发展的理解。
Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer. This carcinoma is histologically characterized by the presence of clear and abundant cytoplasm. In the present study, we sought to identify genes differentially expressed in ccRCC and build a molecular profile of this cancer. We selected genes described in the literature related to cellular differentiation and proliferation. We analyzed the gene and protein expression by quantitative PCR (qPCR) and immunohistochemistry, respectively, and examined possible epigenetic mechanisms that regulate their expression in ccRCC samples and cell lines. Occludin (OCLN) and growth arrest-specific 1 (GAS1) genes were underexpressed in ccRCC, and we report that miR-122 and miR-34a, respectively, may regulate their expression in this cancer. Furthermore, we showed by qPCR and immunohistochemistry that solute carrier family 2 member 1 (SLC2A1) was significantly overexpressed in ccRCC. The set of genes identified in the present study furthers our understanding of the molecular basis and development of ccRCC.