An Improved Media System for High Regeneration Rates from Barley Immature Embryo-Derived Callus Cultures of Commercial Cultivars
An Improved Media System for High Regeneration Rates from Barley Immature Embryo-Derived Callus Cultures of Commercial Cultivars
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DOI：
10.2135/cropsci2002.9340
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发表时间：
2002-05
期刊：
影响因子：
2.3
通讯作者：
L. Dahleen;P. Bregitzer
中科院分区：
文献类型：
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作者：
L. Dahleen;P. Bregitzer
优良大麦品种（Hordeum vulgare L.）会促进它们的基因转化通过改变铁和硼酸的浓度，以及在培养基中加入6-苄基氨基嘌呤（BAP），显著提高了从“Morex”和“哈灵顿”的组织再生绿色植株的能力。对不同浓度的8种微量营养元素进行植株再生试验，结果表明：KH 2 PO 4、NaMoO 4、MnSO 4、KI、CoCl 2和ZnSO 4以MS浓度为宜。增加H3 BO 3（0.75 mM）和减少FeSO 4（0.05 mM）促进绿色植株再生.在维持培养基中添加0.1 mg/L BAP可提高再生率。这些变化的影响，结合以前报道的改进，通过比较再生反应的8个品种和优良育种系的改进媒体与原来的MS为基础的媒体进行了评估。绿色植株再生对于所有测试的品系和栽培品种都得到改善，从平均每个培养皿2个绿色植株到29个绿色植株。从所有的精英线和品种的再生率超过了原来的媒体上的高度可再生的“黄金承诺”，表明穷人的植物再生不应再限制现代北美品种的转化。
Improved plant regeneration from tissues of elite barley cultivars (Hordeum vulgare L.) will facilitate their genetic transformation. Significant improvements in green plant regeneration from tissues of 'Morex' and Harrington' were achieved by modifying iron and boric acid concentrations, and by adding 6-benzyl aminopurine (BAP) to the culture medium. The plant regeneration in response to various concentrations of eight micronutrients indicated that MS concentrations were appropriate for KH 2 PO 4 , NaMoO 4 , MnSO 4 , KI, CoCl 2 , and ZnSO 4 . Increased H 3 BO 3 (0.75 mM) and decreased FeSO 4 (0.05 mM) improved green plant regeneration. The addition of 0.1 mg/L BAP to maintenance media resulted in an increase in regeneration. The effect of these changes, in combination with previously reported improvements, was assessed by comparing regeneration responses of eight cultivars and elite breeding lines on the improved media versus the original MS-based media. Green plant regeneration was improved for all tested lines and cultivars, from an average of two green plants to 29 green plants per Petri dish. Regeneration rates from all elite lines and cultivars on the improved media exceeded that of the highly regenerable 'Golden Promise' on the original media, indicating that poor plant regeneration should no longer limit the transformation of modern North American cultivars.