Increased leucine-rich repeats and immunoglobulin-like domains 1 expression enhances chemosensitivity in glioma
Increased leucine-rich repeats and immunoglobulin-like domains 1 expression enhances chemosensitivity in glioma
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富含亮氨酸重复序列和免疫球蛋白样结构域 1 表达的增加可增强神经胶质瘤的化疗敏感性
DOI：
10.3969/j.issn.1673-5374.2011.32.007
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发表时间：
2011-11-15
影响因子：
6.1
通讯作者：
Yan, Mingmin
中科院分区：
文献类型：
--
作者：
Liu, Baohui;Chen, Qianxue;Yan, Mingmin
富含亮氨酸重复序列和免疫球蛋白样结构域1（LRIG 1）是一种抑癌基因。室管膜细胞中LRIG 1与Bcl-2相关。Bcl-2和锰超氧化物歧化酶表达降低可提高胶质瘤的环境敏感性。本研究构建了人脑星形细胞瘤组织芯片。为探讨LRIG 1、Bcl-2和锰超氧化物歧化酶（MnSOD）的关系，采用免疫组织化学方法检测组织芯片中LRIG 1、Bcl-2和MnSOD的表达。此外，我们还构建了LRIG 1-U251细胞株，并采用MTT法检测其对阿霉素和替莫唑胺的反应。结果显示，LRIG 1在胶质瘤中的表达与Bcl-2、锰超氧化物歧化酶的表达呈显著负相关。此外，暴露于多柔比星或替莫唑胺的LRIG 1-U251细胞的增殖被显著抑制，即在LRIG 1-U251细胞系中，对多柔比星和替莫唑胺的化学敏感性增加。这表明增加的LRIG 1表达在胶质瘤中产生化学敏感性。
Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) is an anti-oncogene. LRIG1 is correlated with Bcl-2 in ependymonnas. Decreased Bcl-2 and manganese superoxide dismutase expression can improve the chennosensitivity of glioma. In the present study, a tissue microarray of human brain astrocytomas was constructed. To investigate the relationship of LRIG1 with Bcl-2 and manganese superoxide dismutase, LRIG1, Bcl-2 and manganese superoxide dismutase expression in our tissue microarray was determined using immunohistochemistry. In addition, we constructed the LRIG1-U251 cell line, and its responses to doxorubicin and temozolomide were detected using the MTT assay. Results showed that LRIG1 expression was significantly negatively correlated with Bcl-2 and manganese superoxide dismutase expression in glioma. Also, proliferation of LRIG1-U251 cells exposed to doxorubicin or temozolomide was significantly inhibited, i.e. in the LRIG1-U251 cell line, the chemosensitivity to doxorubicin and temozolomide was increased. This indicates that increased LRIG1 expression produces a chemosensitivity in glioma.